Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13294 | 5' | -53.3 | NC_003409.1 | + | 96858 | 0.66 | 0.950708 |
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Target: 5'- aGGGGGCACGCuu---CUGgCGGAGg- -3' miRNA: 3'- -CUCCUGUGCGucuauGAUgGCCUCac -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 44743 | 0.66 | 0.946342 |
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Target: 5'- aAGGAgGCGCAcGGccACUACCGGAa-- -3' miRNA: 3'- cUCCUgUGCGU-CUa-UGAUGGCCUcac -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 27255 | 0.67 | 0.936866 |
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Target: 5'- -uGGACGgGCgguGGGUGCUGCUGGAa-- -3' miRNA: 3'- cuCCUGUgCG---UCUAUGAUGGCCUcac -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 122702 | 0.67 | 0.926385 |
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Target: 5'- uGAGGugGCGCGGAaACUggGCaCGGAugacagggaaGUGg -3' miRNA: 3'- -CUCCugUGCGUCUaUGA--UG-GCCU----------CAC- -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 82395 | 0.67 | 0.920766 |
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Target: 5'- -cGGAUGCGCucgu-CUGCCGGAGg- -3' miRNA: 3'- cuCCUGUGCGucuauGAUGGCCUCac -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 19650 | 0.69 | 0.874485 |
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Target: 5'- cAGGGCACGCGuccagGCaACCGGGGUc -3' miRNA: 3'- cUCCUGUGCGUcua--UGaUGGCCUCAc -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 42672 | 0.7 | 0.817093 |
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Target: 5'- aGAGcGAUACGCAGAcUACUcCUGGGGg- -3' miRNA: 3'- -CUC-CUGUGCGUCU-AUGAuGGCCUCac -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 4584 | 0.73 | 0.648186 |
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Target: 5'- aGAGGACGCGUAGGccaGgUGCUGGAGg- -3' miRNA: 3'- -CUCCUGUGCGUCUa--UgAUGGCCUCac -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 24738 | 0.81 | 0.27629 |
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Target: 5'- aGGGGGCGCGCcggggcuccugGGGUGCUGCCGGGGc- -3' miRNA: 3'- -CUCCUGUGCG-----------UCUAUGAUGGCCUCac -5' |
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| 13294 | 5' | -53.3 | NC_003409.1 | + | 40275 | 1.08 | 0.005246 |
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Target: 5'- cGAGGACACGCAGAUACUACCGGAGUGc -3' miRNA: 3'- -CUCCUGUGCGUCUAUGAUGGCCUCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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