Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13299 | 5' | -54.6 | NC_003409.1 | + | 48793 | 0.67 | 0.901173 |
Target: 5'- gGCCagggGUCAcAGUUUCcagGCCGAGcUAGGUa -3' miRNA: 3'- -CGG----CGGUuUCAGAGa--CGGCUC-AUCCG- -5' |
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13299 | 5' | -54.6 | NC_003409.1 | + | 67041 | 0.66 | 0.918317 |
Target: 5'- cGUCGCCAccuucgaGGGUCUCUGUCaucuuGGUgacguuauuuuccAGGCa -3' miRNA: 3'- -CGGCGGU-------UUCAGAGACGGc----UCA-------------UCCG- -5' |
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13299 | 5' | -54.6 | NC_003409.1 | + | 28101 | 0.66 | 0.918317 |
Target: 5'- cGCCGCuCAGAagcacacGUCUUcggccaaUGCCGuGUuGGCg -3' miRNA: 3'- -CGGCG-GUUU-------CAGAG-------ACGGCuCAuCCG- -5' |
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13299 | 5' | -54.6 | NC_003409.1 | + | 74556 | 0.66 | 0.919468 |
Target: 5'- uCCGCCGGAGaCUCcggGCCGA--AGGa -3' miRNA: 3'- cGGCGGUUUCaGAGa--CGGCUcaUCCg -5' |
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13299 | 5' | -54.6 | NC_003409.1 | + | 73864 | 0.66 | 0.925077 |
Target: 5'- aGuuGCCAAcaguAGUCccUCUGCCGGccAGcGCg -3' miRNA: 3'- -CggCGGUU----UCAG--AGACGGCUcaUC-CG- -5' |
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13299 | 5' | -54.6 | NC_003409.1 | + | 11911 | 0.66 | 0.935555 |
Target: 5'- uGgCGCCAucuAGUgUUCUGCCGAGcagcuugAGGa -3' miRNA: 3'- -CgGCGGUu--UCA-GAGACGGCUCa------UCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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