Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13308 | 3' | -49.3 | NC_003409.1 | + | 35159 | 0.69 | 0.960884 |
Target: 5'- cAGCGAGCUCAAGGCc--UGUGCc-- -3' miRNA: 3'- cUCGCUCGAGUUUCGuuuGUACGacg -5' |
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13308 | 3' | -49.3 | NC_003409.1 | + | 95421 | 0.69 | 0.960884 |
Target: 5'- aGAGCGcGCUCAcuguccAGGCGGcACAUGgUGUc -3' miRNA: 3'- -CUCGCuCGAGU------UUCGUU-UGUACgACG- -5' |
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13308 | 3' | -49.3 | NC_003409.1 | + | 115586 | 0.7 | 0.948343 |
Target: 5'- --aCGAGCUCAGAGCuccuuGACGaGCcGCg -3' miRNA: 3'- cucGCUCGAGUUUCGu----UUGUaCGaCG- -5' |
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13308 | 3' | -49.3 | NC_003409.1 | + | 108247 | 0.71 | 0.922048 |
Target: 5'- cGAGCGA-CUCGAAGUccGCccGCUGUa -3' miRNA: 3'- -CUCGCUcGAGUUUCGuuUGuaCGACG- -5' |
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13308 | 3' | -49.3 | NC_003409.1 | + | 44775 | 0.72 | 0.888901 |
Target: 5'- aGGCGAGCUCAucgcccuuGAGCAGGCGcuucucaaGCUcGCg -3' miRNA: 3'- cUCGCUCGAGU--------UUCGUUUGUa-------CGA-CG- -5' |
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13308 | 3' | -49.3 | NC_003409.1 | + | 99481 | 0.73 | 0.856843 |
Target: 5'- uGAGCGcaguauuGGCguggCGcAGCAcACGUGCUGCg -3' miRNA: 3'- -CUCGC-------UCGa---GUuUCGUuUGUACGACG- -5' |
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13308 | 3' | -49.3 | NC_003409.1 | + | 54778 | 1.13 | 0.005066 |
Target: 5'- cGAGCGAGCUCAAAGCAAACAUGCUGCu -3' miRNA: 3'- -CUCGCUCGAGUUUCGUUUGUACGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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