Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13309 | 3' | -53.7 | NC_003409.1 | + | 9946 | 0.66 | 0.951637 |
Target: 5'- cACGUcaUCGCCGCCccCCUCcGCAUCc- -3' miRNA: 3'- -UGCAaaAGUGGUGGa-GGAG-CGUGGcg -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 3356 | 0.66 | 0.951637 |
Target: 5'- --aUUUUCACUACCUCUuUUGCACgGg -3' miRNA: 3'- ugcAAAAGUGGUGGAGG-AGCGUGgCg -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 133478 | 0.66 | 0.955666 |
Target: 5'- uGCGcaccaUCGCCACCacUCCUgGCAaCGCc -3' miRNA: 3'- -UGCaaa--AGUGGUGG--AGGAgCGUgGCG- -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 67970 | 0.66 | 0.955666 |
Target: 5'- uCGUgg-CGCC-CC-CUgCGCGCCGCa -3' miRNA: 3'- uGCAaaaGUGGuGGaGGaGCGUGGCG- -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 102483 | 0.66 | 0.963026 |
Target: 5'- cGCGUguaCGCCGCgCUgcCCUCGCAacucaUGCg -3' miRNA: 3'- -UGCAaaaGUGGUG-GA--GGAGCGUg----GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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