Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13309 | 3' | -53.7 | NC_003409.1 | + | 32750 | 0.68 | 0.891215 |
Target: 5'- uGCGUcccaggACCACCUCgUCGUAggucCCGCa -3' miRNA: 3'- -UGCAaaag--UGGUGGAGgAGCGU----GGCG- -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 109615 | 0.7 | 0.829451 |
Target: 5'- uGCGUg--CGCCGCCUCCgcgUGCgaagGCUGUu -3' miRNA: 3'- -UGCAaaaGUGGUGGAGGa--GCG----UGGCG- -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 46103 | 0.7 | 0.820818 |
Target: 5'- -----gUCACCGCgUCCgaacguaccUCGCGCCGUa -3' miRNA: 3'- ugcaaaAGUGGUGgAGG---------AGCGUGGCG- -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 38138 | 0.71 | 0.784598 |
Target: 5'- uAUGUccggCGCCGCCUCUUgaGCugCGCa -3' miRNA: 3'- -UGCAaaa-GUGGUGGAGGAg-CGugGCG- -5' |
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13309 | 3' | -53.7 | NC_003409.1 | + | 133478 | 0.66 | 0.955666 |
Target: 5'- uGCGcaccaUCGCCACCacUCCUgGCAaCGCc -3' miRNA: 3'- -UGCaaa--AGUGGUGG--AGGAgCGUgGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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