Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1332 | 3' | -61.9 | NC_001335.1 | + | 22779 | 0.66 | 0.399785 |
Target: 5'- gACAGCCaCCgacuaccagaagaugACGuuGgUCGUCGGGACu -3' miRNA: 3'- gUGUCGG-GG---------------UGCggC-AGCGGUCCUGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 17243 | 0.66 | 0.387609 |
Target: 5'- uGCAGCaccgcgccugcgaCCugGCCGagggccgucgccagCGCCGGGAUGu -3' miRNA: 3'- gUGUCGg------------GGugCGGCa-------------GCGGUCCUGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 51833 | 0.66 | 0.387608 |
Target: 5'- ---cGCCCCGuCGCUGUCGCUGgucucGGugGc -3' miRNA: 3'- guguCGGGGU-GCGGCAGCGGU-----CCugC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 23325 | 0.66 | 0.379062 |
Target: 5'- gACAGCUCCAcCGCCuG-CGCCgguAGGAUc -3' miRNA: 3'- gUGUCGGGGU-GCGG-CaGCGG---UCCUGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 36836 | 0.66 | 0.379062 |
Target: 5'- gGCGGUUCCuggaGCCGcugCGCCAGGucuACGa -3' miRNA: 3'- gUGUCGGGGug--CGGCa--GCGGUCC---UGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 2258 | 0.66 | 0.370643 |
Target: 5'- cCACcGCCCCACuucgugGCCGacaGCCGGGuCa -3' miRNA: 3'- -GUGuCGGGGUG------CGGCag-CGGUCCuGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 23091 | 0.66 | 0.369807 |
Target: 5'- aACGGCCCCGUGCUG--GCCAcauggucGGACGa -3' miRNA: 3'- gUGUCGGGGUGCGGCagCGGU-------CCUGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 34114 | 0.66 | 0.362352 |
Target: 5'- gGCAGCUUCGCgGUCGccCGCgAGGACa -3' miRNA: 3'- gUGUCGGGGUG-CGGCa-GCGgUCCUGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 28082 | 0.66 | 0.362351 |
Target: 5'- gGCAGUCCuCGgGgaCGUUGUCGGGGCGu -3' miRNA: 3'- gUGUCGGG-GUgCg-GCAGCGGUCCUGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 45489 | 0.67 | 0.34616 |
Target: 5'- gACgAG-CCCGCGCCGuUCGagCAGGAUGa -3' miRNA: 3'- gUG-UCgGGGUGCGGC-AGCg-GUCCUGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 39727 | 0.67 | 0.34616 |
Target: 5'- -uCAGCgCCGCGagCGcCGCCGuGGACGa -3' miRNA: 3'- guGUCGgGGUGCg-GCaGCGGU-CCUGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 25790 | 0.67 | 0.322858 |
Target: 5'- uGCAGCggucggaaCCCuCGCCGUCGgCGGGcugGCGu -3' miRNA: 3'- gUGUCG--------GGGuGCGGCAGCgGUCC---UGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 49350 | 0.67 | 0.315356 |
Target: 5'- gGCAGUCCCacGCGCUGUaaCCAGGGa- -3' miRNA: 3'- gUGUCGGGG--UGCGGCAgcGGUCCUgc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 20341 | 0.67 | 0.314613 |
Target: 5'- aCACAucgacGCCcucgaCCugGCCGUCGCCGgccaguuGGACc -3' miRNA: 3'- -GUGU-----CGG-----GGugCGGCAGCGGU-------CCUGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 2792 | 0.67 | 0.307985 |
Target: 5'- gACGGCCgCACGCaccaGcUCGUCAaGGACa -3' miRNA: 3'- gUGUCGGgGUGCGg---C-AGCGGU-CCUGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 32921 | 0.67 | 0.307985 |
Target: 5'- gCGguGCUCCACgGUCGUCG-UAGGACa -3' miRNA: 3'- -GUguCGGGGUG-CGGCAGCgGUCCUGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 688 | 0.68 | 0.293641 |
Target: 5'- gCGCuGCaCUCACGCCGgauuucaGCCucuGGACGa -3' miRNA: 3'- -GUGuCG-GGGUGCGGCag-----CGGu--CCUGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 18608 | 0.68 | 0.279824 |
Target: 5'- gCGCGGCCCCugcuuCG-CGUCGUUgAGGAUGa -3' miRNA: 3'- -GUGUCGGGGu----GCgGCAGCGG-UCCUGC- -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 7063 | 0.68 | 0.265877 |
Target: 5'- -uCAGCCgcguggaucaccgUCGCgGCCGUCaGCCAGGACc -3' miRNA: 3'- guGUCGG-------------GGUG-CGGCAG-CGGUCCUGc -5' |
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1332 | 3' | -61.9 | NC_001335.1 | + | 37900 | 0.68 | 0.260075 |
Target: 5'- -cCGGCgUCGuCGCCGUCgGCCAGGAg- -3' miRNA: 3'- guGUCGgGGU-GCGGCAG-CGGUCCUgc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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