Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1332 | 3' | -61.9 | NC_001335.1 | + | 3184 | 0.69 | 0.224001 |
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Target: 5'- cCAuCAGCUCC-CGCCGcUCGCCuuGGCGa -3' miRNA: 3'- -GU-GUCGGGGuGCGGC-AGCGGucCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 32943 | 0.7 | 0.218419 |
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Target: 5'- cCugAGCCugagaCCGCGCCGUCGgcgaCGGuGACGa -3' miRNA: 3'- -GugUCGG-----GGUGCGGCAGCg---GUC-CUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 26491 | 0.71 | 0.177928 |
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Target: 5'- cCGCAGCCCacuguCAUGCC--UGUCAGGACGa -3' miRNA: 3'- -GUGUCGGG-----GUGCGGcaGCGGUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 24159 | 0.72 | 0.140468 |
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Target: 5'- -cCAGCCCC--GCCGcCGCCAGGAg- -3' miRNA: 3'- guGUCGGGGugCGGCaGCGGUCCUgc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 25908 | 0.74 | 0.099019 |
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Target: 5'- gACGGCCUgACGCuCGUCGCCAcGGuCGc -3' miRNA: 3'- gUGUCGGGgUGCG-GCAGCGGU-CCuGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 23820 | 0.77 | 0.065577 |
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Target: 5'- cCACcucuGCCCCA-GCCGUCaGCCAGGGCc -3' miRNA: 3'- -GUGu---CGGGGUgCGGCAG-CGGUCCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 42043 | 0.77 | 0.063784 |
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Target: 5'- --gAGUCCCACGCCuUCGCCAGGGUGg -3' miRNA: 3'- gugUCGGGGUGCGGcAGCGGUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 1900 | 1.08 | 0.000253 |
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Target: 5'- cCACAGCCCCACGCCGUCGCCAGGACGg -3' miRNA: 3'- -GUGUCGGGGUGCGGCAGCGGUCCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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