Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1332 | 3' | -61.9 | NC_001335.1 | + | 34114 | 0.66 | 0.362352 |
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Target: 5'- gGCAGCUUCGCgGUCGccCGCgAGGACa -3' miRNA: 3'- gUGUCGGGGUG-CGGCa-GCGgUCCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 23091 | 0.66 | 0.369807 |
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Target: 5'- aACGGCCCCGUGCUG--GCCAcauggucGGACGa -3' miRNA: 3'- gUGUCGGGGUGCGGCagCGGU-------CCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 2258 | 0.66 | 0.370643 |
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Target: 5'- cCACcGCCCCACuucgugGCCGacaGCCGGGuCa -3' miRNA: 3'- -GUGuCGGGGUG------CGGCag-CGGUCCuGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 36836 | 0.66 | 0.379062 |
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Target: 5'- gGCGGUUCCuggaGCCGcugCGCCAGGucuACGa -3' miRNA: 3'- gUGUCGGGGug--CGGCa--GCGGUCC---UGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 23325 | 0.66 | 0.379062 |
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Target: 5'- gACAGCUCCAcCGCCuG-CGCCgguAGGAUc -3' miRNA: 3'- gUGUCGGGGU-GCGG-CaGCGG---UCCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 51833 | 0.66 | 0.387608 |
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Target: 5'- ---cGCCCCGuCGCUGUCGCUGgucucGGugGc -3' miRNA: 3'- guguCGGGGU-GCGGCAGCGGU-----CCugC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 17243 | 0.66 | 0.387609 |
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Target: 5'- uGCAGCaccgcgccugcgaCCugGCCGagggccgucgccagCGCCGGGAUGu -3' miRNA: 3'- gUGUCGg------------GGugCGGCa-------------GCGGUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 22779 | 0.66 | 0.399785 |
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Target: 5'- gACAGCCaCCgacuaccagaagaugACGuuGgUCGUCGGGACu -3' miRNA: 3'- gUGUCGG-GG---------------UGCggC-AGCGGUCCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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