Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1332 | 3' | -61.9 | NC_001335.1 | + | 34114 | 0.66 | 0.362352 |
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Target: 5'- gGCAGCUUCGCgGUCGccCGCgAGGACa -3' miRNA: 3'- gUGUCGGGGUG-CGGCa-GCGgUCCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 36836 | 0.66 | 0.379062 |
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Target: 5'- gGCGGUUCCuggaGCCGcugCGCCAGGucuACGa -3' miRNA: 3'- gUGUCGGGGug--CGGCa--GCGGUCC---UGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 37900 | 0.68 | 0.260075 |
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Target: 5'- -cCGGCgUCGuCGCCGUCgGCCAGGAg- -3' miRNA: 3'- guGUCGgGGU-GCGGCAG-CGGUCCUgc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 39727 | 0.67 | 0.34616 |
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Target: 5'- -uCAGCgCCGCGagCGcCGCCGuGGACGa -3' miRNA: 3'- guGUCGgGGUGCg-GCaGCGGU-CCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 42043 | 0.77 | 0.063784 |
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Target: 5'- --gAGUCCCACGCCuUCGCCAGGGUGg -3' miRNA: 3'- gugUCGGGGUGCGGcAGCGGUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 45489 | 0.67 | 0.34616 |
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Target: 5'- gACgAG-CCCGCGCCGuUCGagCAGGAUGa -3' miRNA: 3'- gUG-UCgGGGUGCGGC-AGCg-GUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 49350 | 0.67 | 0.315356 |
|
Target: 5'- gGCAGUCCCacGCGCUGUaaCCAGGGa- -3' miRNA: 3'- gUGUCGGGG--UGCGGCAgcGGUCCUgc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 51833 | 0.66 | 0.387608 |
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Target: 5'- ---cGCCCCGuCGCUGUCGCUGgucucGGugGc -3' miRNA: 3'- guguCGGGGU-GCGGCAGCGGU-----CCugC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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