Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1332 | 3' | -61.9 | NC_001335.1 | + | 18608 | 0.68 | 0.279824 |
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Target: 5'- gCGCGGCCCCugcuuCG-CGUCGUUgAGGAUGa -3' miRNA: 3'- -GUGUCGGGGu----GCgGCAGCGG-UCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 17243 | 0.66 | 0.387609 |
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Target: 5'- uGCAGCaccgcgccugcgaCCugGCCGagggccgucgccagCGCCGGGAUGu -3' miRNA: 3'- gUGUCGg------------GGugCGGCa-------------GCGGUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 7063 | 0.68 | 0.265877 |
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Target: 5'- -uCAGCCgcguggaucaccgUCGCgGCCGUCaGCCAGGACc -3' miRNA: 3'- guGUCGG-------------GGUG-CGGCAG-CGGUCCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 3184 | 0.69 | 0.224001 |
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Target: 5'- cCAuCAGCUCC-CGCCGcUCGCCuuGGCGa -3' miRNA: 3'- -GU-GUCGGGGuGCGGC-AGCGGucCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 2792 | 0.67 | 0.307985 |
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Target: 5'- gACGGCCgCACGCaccaGcUCGUCAaGGACa -3' miRNA: 3'- gUGUCGGgGUGCGg---C-AGCGGU-CCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 2258 | 0.66 | 0.370643 |
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Target: 5'- cCACcGCCCCACuucgugGCCGacaGCCGGGuCa -3' miRNA: 3'- -GUGuCGGGGUG------CGGCag-CGGUCCuGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 1900 | 1.08 | 0.000253 |
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Target: 5'- cCACAGCCCCACGCCGUCGCCAGGACGg -3' miRNA: 3'- -GUGUCGGGGUGCGGCAGCGGUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 688 | 0.68 | 0.293641 |
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Target: 5'- gCGCuGCaCUCACGCCGgauuucaGCCucuGGACGa -3' miRNA: 3'- -GUGuCG-GGGUGCGGCag-----CGGu--CCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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