Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13322 | 5' | -53.3 | NC_003409.1 | + | 118315 | 0.71 | 0.765474 |
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Target: 5'- gGGgUGCgCCGCGCCGGguuccugGggUGCGCCGc -3' miRNA: 3'- -CUgACG-GGCGUGGUUa------UuuGCGUGGC- -5' |
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| 13322 | 5' | -53.3 | NC_003409.1 | + | 111326 | 0.71 | 0.765474 |
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Target: 5'- --gUGCCCGCcguuACCccaAGGCGCACCGu -3' miRNA: 3'- cugACGGGCG----UGGuuaUUUGCGUGGC- -5' |
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| 13322 | 5' | -53.3 | NC_003409.1 | + | 102443 | 0.71 | 0.745739 |
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Target: 5'- aGCUGCCCagGCACCGGUAGAaauaGUGuCCGa -3' miRNA: 3'- cUGACGGG--CGUGGUUAUUUg---CGU-GGC- -5' |
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| 13322 | 5' | -53.3 | NC_003409.1 | + | 88266 | 0.73 | 0.642321 |
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Target: 5'- aGGCUGCCUGCuccguguggauACCAGUGAaugaggGCGCAUCu -3' miRNA: 3'- -CUGACGGGCG-----------UGGUUAUU------UGCGUGGc -5' |
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| 13322 | 5' | -53.3 | NC_003409.1 | + | 101826 | 0.76 | 0.507515 |
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Target: 5'- --aUGCCCGUAuCCAAaGAACGCGCCc -3' miRNA: 3'- cugACGGGCGU-GGUUaUUUGCGUGGc -5' |
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| 13322 | 5' | -53.3 | NC_003409.1 | + | 80584 | 1.08 | 0.005105 |
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Target: 5'- uGACUGCCCGCACCAAUAAACGCACCGc -3' miRNA: 3'- -CUGACGGGCGUGGUUAUUUGCGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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