miRNA: 3'- gaCUGCugUua---UUGUGUCCCGGuCCu -5'
miRNA: 3'- -GACu---GCUGUuauUGUG---UCCCGGUCCU- -5'
miRNA: 3'- -GACU--GCu--GUUAUUgugUCCCGGUCCU- -5'
miRNA: 3'- gaCUGCUGuuAUUGUGU-CCCGGUcCU- -5'
miRNA: 3'- gACUGCUGUuauuGUGUCCcGGUCCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13328 | 3' | -51.7 | NC_003409.1 | + | 111990 | 0.68 | 0.948354 |
|
Target: 5'- -gGGCGugGugccgGAUGCAGGGCCuGGc -3' miRNA: 3'- gaCUGCugUua---UUGUGUCCCGGuCCu -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 112147 | 0.68 | 0.943799 |
|
Target: 5'- cCUGcaucCGGCAcc-ACGCccgGGGGCCAGGAc -3' miRNA: 3'- -GACu---GCUGUuauUGUG---UCCCGGUCCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 119069 | 0.66 | 0.978611 |
|
Target: 5'- aCUGGguCGuuuCAGUAGaugGGGGCCAGGAu -3' miRNA: 3'- -GACU--GCu--GUUAUUgugUCCCGGUCCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 120217 | 0.67 | 0.964098 |
|
Target: 5'- -aGGCGGC--UGGCACAcGGGCCGuGAg -3' miRNA: 3'- gaCUGCUGuuAUUGUGU-CCCGGUcCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 128317 | 0.67 | 0.964098 |
|
Target: 5'- gUGGCGACGuu--UACGGGaCCGGGGa -3' miRNA: 3'- gACUGCUGUuauuGUGUCCcGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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