miRNA: 3'- -GACU--GCu--GUUAUUgugUCCCGGUCCU- -5'
miRNA: 3'- gACuGCUgu--------UAUUGUGUCCCGGUCCU- -5'
miRNA: 3'- -GACugCUGuuauuGUG---UCCCGGUCCU- -5'
miRNA: 3'- gaCUGCUGuua-----UUGuGUCC-CGGUCCu -5'
miRNA: 3'- gaCUGCUG---------UUA---UUGuGUCCCGGUCCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13328 | 3' | -51.7 | NC_003409.1 | + | 119069 | 0.66 | 0.978611 |
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Target: 5'- aCUGGguCGuuuCAGUAGaugGGGGCCAGGAu -3' miRNA: 3'- -GACU--GCu--GUUAUUgugUCCCGGUCCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 109042 | 0.66 | 0.980894 |
|
Target: 5'- uUGuCGAaaggucccgcGUAAUGCAGGGCUGGGAu -3' miRNA: 3'- gACuGCUgu--------UAUUGUGUCCCGGUCCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 96776 | 0.66 | 0.980894 |
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Target: 5'- cCUGugGAUuuu--CACuauGGGGUCAGGGu -3' miRNA: 3'- -GACugCUGuuauuGUG---UCCCGGUCCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 63978 | 0.66 | 0.98299 |
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Target: 5'- -aGACGACcacucaggGGCuCAGGcGCCAGGu -3' miRNA: 3'- gaCUGCUGuua-----UUGuGUCC-CGGUCCu -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 77067 | 0.66 | 0.984908 |
|
Target: 5'- -cGGCGGCcgcuaagaaAAUcgaAGC-CAGGGUCAGGAc -3' miRNA: 3'- gaCUGCUG---------UUA---UUGuGUCCCGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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