miRNA: 3'- -GACUGCUGUuAuuGUGUCC--------CGGU-CCU- -5'
miRNA: 3'- gAC-UGCUGUuauUGUgUCCCGGUCCU- -5'
miRNA: 3'- gACUGCUGUuauUGUGuCCC-GGUCCU- -5'
miRNA: 3'- -GACUGCUGuuauUGUGUCCCGGUCcu -5'
miRNA: 3'- -GACUGCUGUUAUUGUGUCCCGGUCCU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13328 | 3' | -51.7 | NC_003409.1 | + | 51005 | 0.7 | 0.876806 |
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Target: 5'- aCUGGCGACAcUggUACAGGcaaaacacGCCGuGGAa -3' miRNA: 3'- -GACUGCUGUuAuuGUGUCC--------CGGU-CCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 84537 | 0.72 | 0.800887 |
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Target: 5'- uUGuACGACG---GCAgAGGGUCAGGAg -3' miRNA: 3'- gAC-UGCUGUuauUGUgUCCCGGUCCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 68075 | 0.74 | 0.701317 |
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Target: 5'- aUGGCGGCAu--ACACuGGGaCCGGGGc -3' miRNA: 3'- gACUGCUGUuauUGUGuCCC-GGUCCU- -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 16792 | 0.76 | 0.584781 |
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Target: 5'- cCUGGCGGCc---GCGCGGGGCCAGc- -3' miRNA: 3'- -GACUGCUGuuauUGUGUCCCGGUCcu -5' |
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| 13328 | 3' | -51.7 | NC_003409.1 | + | 90003 | 1.1 | 0.00554 |
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Target: 5'- cCUGACGACAAUAACACAGGGCCAGGAg -3' miRNA: 3'- -GACUGCUGUUAUUGUGUCCCGGUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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