miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1335 3' -56.1 NC_001335.1 + 2656 0.66 0.614229
Target:  5'- -uUCGGGUggucgcuuccuccggGCUCUaaguucccGCCGUcGGGCUACg -3'
miRNA:   3'- ucAGCCCG---------------CGAGA--------CGGUAaCUCGAUG- -5'
1335 3' -56.1 NC_001335.1 + 8868 0.67 0.576479
Target:  5'- -aUCGGGaCGUUCUGaucccaacCCAggucGAGCUGCg -3'
miRNA:   3'- ucAGCCC-GCGAGAC--------GGUaa--CUCGAUG- -5'
1335 3' -56.1 NC_001335.1 + 5275 0.68 0.500807
Target:  5'- gAG-CGGcGCGCUCgcgcagGCgAUcucggUGAGCUGCa -3'
miRNA:   3'- -UCaGCC-CGCGAGa-----CGgUA-----ACUCGAUG- -5'
1335 3' -56.1 NC_001335.1 + 31614 0.72 0.270709
Target:  5'- uGUUGGGUGCgaUCUGUCg--GAGCUGCc -3'
miRNA:   3'- uCAGCCCGCG--AGACGGuaaCUCGAUG- -5'
1335 3' -56.1 NC_001335.1 + 4280 1.1 0.000524
Target:  5'- cAGUCGGGCGCUCUGCCAUUGAGCUACa -3'
miRNA:   3'- -UCAGCCCGCGAGACGGUAACUCGAUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.