Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1335 | 3' | -56.1 | NC_001335.1 | + | 31614 | 0.72 | 0.270709 |
Target: 5'- uGUUGGGUGCgaUCUGUCg--GAGCUGCc -3' miRNA: 3'- uCAGCCCGCG--AGACGGuaaCUCGAUG- -5' |
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1335 | 3' | -56.1 | NC_001335.1 | + | 5275 | 0.68 | 0.500807 |
Target: 5'- gAG-CGGcGCGCUCgcgcagGCgAUcucggUGAGCUGCa -3' miRNA: 3'- -UCaGCC-CGCGAGa-----CGgUA-----ACUCGAUG- -5' |
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1335 | 3' | -56.1 | NC_001335.1 | + | 2656 | 0.66 | 0.614229 |
Target: 5'- -uUCGGGUggucgcuuccuccggGCUCUaaguucccGCCGUcGGGCUACg -3' miRNA: 3'- ucAGCCCG---------------CGAGA--------CGGUAaCUCGAUG- -5' |
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1335 | 3' | -56.1 | NC_001335.1 | + | 8868 | 0.67 | 0.576479 |
Target: 5'- -aUCGGGaCGUUCUGaucccaacCCAggucGAGCUGCg -3' miRNA: 3'- ucAGCCC-GCGAGAC--------GGUaa--CUCGAUG- -5' |
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1335 | 3' | -56.1 | NC_001335.1 | + | 4280 | 1.1 | 0.000524 |
Target: 5'- cAGUCGGGCGCUCUGCCAUUGAGCUACa -3' miRNA: 3'- -UCAGCCCGCGAGACGGUAACUCGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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