Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13358 | 5' | -57.4 | NC_003409.1 | + | 115148 | 0.68 | 0.734423 |
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Target: 5'- uGCugGaCCUCgACCAugGCGgcaGCGgcagaCCu -3' miRNA: 3'- -UGugC-GGAGgUGGUugCGCa--CGCa----GG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 116306 | 0.69 | 0.704955 |
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Target: 5'- cGCACcCCaUCCACCGAUGCcgaaaucaGUGUCCa -3' miRNA: 3'- -UGUGcGG-AGGUGGUUGCGca------CGCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 117676 | 0.7 | 0.613196 |
|
Target: 5'- gUugGCCUCCAgCAaagcacauuuugcACGCaaaugGCGUCCg -3' miRNA: 3'- uGugCGGAGGUgGU-------------UGCGca---CGCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 127942 | 0.69 | 0.664858 |
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Target: 5'- cCGC-CCUCCACU-ACGCG-GCGcCCg -3' miRNA: 3'- uGUGcGGAGGUGGuUGCGCaCGCaGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 130864 | 0.68 | 0.753648 |
|
Target: 5'- gUugGUCUCCAgguuuaccgcCCAGCGCuggggugguGUGgGUCCg -3' miRNA: 3'- uGugCGGAGGU----------GGUUGCG---------CACgCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 132965 | 0.75 | 0.37197 |
|
Target: 5'- aGCGCGCCgCgCGCCuguGCGCGUGCcagguaacgGUCCu -3' miRNA: 3'- -UGUGCGGaG-GUGGu--UGCGCACG---------CAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 134820 | 1.12 | 0.001348 |
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Target: 5'- aACACGCCUCCACCAACGCGUGCGUCCg -3' miRNA: 3'- -UGUGCGGAGGUGGUUGCGCACGCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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