Results 41 - 47 of 47 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13358 | 5' | -57.4 | NC_003409.1 | + | 86023 | 0.69 | 0.664858 |
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Target: 5'- cGCACuGCCgCCACCc-CGCGgaagauugGUGUCCa -3' miRNA: 3'- -UGUG-CGGaGGUGGuuGCGCa-------CGCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 52503 | 0.69 | 0.673935 |
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Target: 5'- gGCACGCCUCUAaaAAgUGCGauuuaacUGCGUCUg -3' miRNA: 3'- -UGUGCGGAGGUggUU-GCGC-------ACGCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 91121 | 0.69 | 0.678967 |
|
Target: 5'- -gGCGCCUCCuguguGCCGAgGUaaaggaaugcuggugGUGCGUUCa -3' miRNA: 3'- ugUGCGGAGG-----UGGUUgCG---------------CACGCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 14900 | 0.69 | 0.684992 |
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Target: 5'- cGCGCGUUgCCGCCuGugGCGUuuGCGUCg -3' miRNA: 3'- -UGUGCGGaGGUGG-UugCGCA--CGCAGg -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 55892 | 0.69 | 0.708921 |
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Target: 5'- uCGCGCUUCagugCACCGugGgcccugacggcaugcUGUGCGUCCu -3' miRNA: 3'- uGUGCGGAG----GUGGUugC---------------GCACGCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 110007 | 0.68 | 0.71485 |
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Target: 5'- -gGCGCCaCCGCCucccgcCGCG-GCGUCUu -3' miRNA: 3'- ugUGCGGaGGUGGuu----GCGCaCGCAGG- -5' |
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| 13358 | 5' | -57.4 | NC_003409.1 | + | 111905 | 0.66 | 0.865303 |
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Target: 5'- aAC-CGUCUCCGCCAgggACGCcgauaggGCGgCCa -3' miRNA: 3'- -UGuGCGGAGGUGGU---UGCGca-----CGCaGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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