Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1337 | 3' | -55.6 | NC_001335.1 | + | 47180 | 0.66 | 0.697294 |
Target: 5'- --cCGACgCGGucUGGCC-CGaacUCGGGAGCg -3' miRNA: 3'- aaaGCUG-GCU--GCCGGuGU---AGCCCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 6066 | 0.66 | 0.697294 |
Target: 5'- cUUCGcGCUGGcCGGCUACucgcaGGGAGCc -3' miRNA: 3'- aAAGC-UGGCU-GCCGGUGuag--CCCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 39941 | 0.66 | 0.686375 |
Target: 5'- -gUCG-CaCGACGGCCuGCAgUGGGAGg -3' miRNA: 3'- aaAGCuG-GCUGCCGG-UGUaGCCCUUg -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 47547 | 0.66 | 0.675404 |
Target: 5'- ---aGGgCGGUGGCCACAUC-GGAGCu -3' miRNA: 3'- aaagCUgGCUGCCGGUGUAGcCCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 22689 | 0.66 | 0.675404 |
Target: 5'- --cCGuGCCGACGGCCACcaacugGUCuGGAu- -3' miRNA: 3'- aaaGC-UGGCUGCCGGUG------UAGcCCUug -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 17141 | 0.66 | 0.664391 |
Target: 5'- --aUGAgCGACGGCaGCAUCGGcuggaGAGCc -3' miRNA: 3'- aaaGCUgGCUGCCGgUGUAGCC-----CUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 16075 | 0.66 | 0.653348 |
Target: 5'- -cUUG-UCGACGGUUACGUCGaccGGGACg -3' miRNA: 3'- aaAGCuGGCUGCCGGUGUAGC---CCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 36253 | 0.66 | 0.653348 |
Target: 5'- -gUCGACCGAgaacCGGCCuccgacaAUC-GGAACa -3' miRNA: 3'- aaAGCUGGCU----GCCGGug-----UAGcCCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 3552 | 0.67 | 0.631212 |
Target: 5'- --cCGGcuCCGACGGCUGCGUCcaGAGCg -3' miRNA: 3'- aaaGCU--GGCUGCCGGUGUAGccCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 20021 | 0.67 | 0.62014 |
Target: 5'- --gUGACCGGugaguuguCGGCCAgGaaCGGGAACg -3' miRNA: 3'- aaaGCUGGCU--------GCCGGUgUa-GCCCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 9663 | 0.67 | 0.587027 |
Target: 5'- -gUCGGCCu-UGGCCGCGUaggUGGGAGu -3' miRNA: 3'- aaAGCUGGcuGCCGGUGUA---GCCCUUg -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 29799 | 0.67 | 0.587027 |
Target: 5'- --gCGACCGAC--UCACGUCGGGcACc -3' miRNA: 3'- aaaGCUGGCUGccGGUGUAGCCCuUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 2177 | 0.67 | 0.576054 |
Target: 5'- --cCGGCUGuCGGCCACGaaGuGGGGCg -3' miRNA: 3'- aaaGCUGGCuGCCGGUGUagC-CCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 20257 | 0.68 | 0.565127 |
Target: 5'- --cCGG-CGACGGCCAgGUCGaGGGCg -3' miRNA: 3'- aaaGCUgGCUGCCGGUgUAGCcCUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 17605 | 0.68 | 0.554256 |
Target: 5'- ---aGGCCGAUGGCCAUcagGUUGGcGAGa -3' miRNA: 3'- aaagCUGGCUGCCGGUG---UAGCC-CUUg -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 29043 | 0.68 | 0.554256 |
Target: 5'- --cUGGCCGcaACGGCUACAUCGucAACg -3' miRNA: 3'- aaaGCUGGC--UGCCGGUGUAGCccUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 29014 | 0.68 | 0.522046 |
Target: 5'- -aUgGGCCGGCGGCUGC--CGGuGGACa -3' miRNA: 3'- aaAgCUGGCUGCCGGUGuaGCC-CUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 40696 | 0.69 | 0.511467 |
Target: 5'- --aCG-CCGAC-GUCGCAUCGGaGAACg -3' miRNA: 3'- aaaGCuGGCUGcCGGUGUAGCC-CUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 15125 | 0.69 | 0.490586 |
Target: 5'- cUUCaGCuCGACGGUCACAUCGucGGACa -3' miRNA: 3'- aAAGcUG-GCUGCCGGUGUAGCc-CUUG- -5' |
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1337 | 3' | -55.6 | NC_001335.1 | + | 11582 | 0.69 | 0.490586 |
Target: 5'- cUUCuGGCCGGuCGuGCC-CAUCGGGAu- -3' miRNA: 3'- aAAG-CUGGCU-GC-CGGuGUAGCCCUug -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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