Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1338 | 3' | -54 | NC_001335.1 | + | 10525 | 0.66 | 0.732826 |
Target: 5'- --cUCGCGGAacuUCUCAAGGUCGUGa-- -3' miRNA: 3'- aucAGCGCCU---GGAGUUUCGGCAUgac -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 15158 | 0.66 | 0.732826 |
Target: 5'- --uUCGCGGGCCUUcuGGCCcaGCUu -3' miRNA: 3'- aucAGCGCCUGGAGuuUCGGcaUGAc -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 6056 | 0.66 | 0.732826 |
Target: 5'- -cGUaCGCGGACUUCGcgcuGGCCG-GCUa -3' miRNA: 3'- auCA-GCGCCUGGAGUu---UCGGCaUGAc -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 11457 | 0.66 | 0.721992 |
Target: 5'- cGGUCuGCGcGACCUUggGGUCGUucACg- -3' miRNA: 3'- aUCAG-CGC-CUGGAGuuUCGGCA--UGac -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 356 | 0.66 | 0.721992 |
Target: 5'- ---cCGcCGGAUCUCAAGGCCGcGCc- -3' miRNA: 3'- aucaGC-GCCUGGAGUUUCGGCaUGac -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 47935 | 0.67 | 0.707769 |
Target: 5'- aAGUCGUGGAcauCCUCAAcucaucgaacgaguGGCCGUuCg- -3' miRNA: 3'- aUCAGCGCCU---GGAGUU--------------UCGGCAuGac -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 8010 | 0.67 | 0.655447 |
Target: 5'- --uUCGCGGggaACCUCcccGCCGUACUu -3' miRNA: 3'- aucAGCGCC---UGGAGuuuCGGCAUGAc -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 24456 | 0.67 | 0.655447 |
Target: 5'- uUGGUgaaCGCGGACUUCGAcAGCCGguugccCUGc -3' miRNA: 3'- -AUCA---GCGCCUGGAGUU-UCGGCau----GAC- -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 38360 | 0.69 | 0.588064 |
Target: 5'- gAGUCGCGGGCUggccGGCCGaGCg- -3' miRNA: 3'- aUCAGCGCCUGGaguuUCGGCaUGac -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 16132 | 0.71 | 0.429129 |
Target: 5'- aAGUCGUuccuGGGCCUCAcacGAGCCGc-CUGg -3' miRNA: 3'- aUCAGCG----CCUGGAGU---UUCGGCauGAC- -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 42094 | 0.71 | 0.429129 |
Target: 5'- aGGUUGCGGAUCUCcaucAGCCGcUugUGu -3' miRNA: 3'- aUCAGCGCCUGGAGuu--UCGGC-AugAC- -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 35854 | 0.72 | 0.391066 |
Target: 5'- aAGUCGCGGGCCUU--GGCCGc---- -3' miRNA: 3'- aUCAGCGCCUGGAGuuUCGGCaugac -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 24274 | 0.78 | 0.160853 |
Target: 5'- -cGUCGCGGGCCUCAGAcGCCaucugGUACUu -3' miRNA: 3'- auCAGCGCCUGGAGUUU-CGG-----CAUGAc -5' |
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1338 | 3' | -54 | NC_001335.1 | + | 6376 | 1.06 | 0.001839 |
Target: 5'- gUAGUCGCGGACCUCAAAGCCGUACUGc -3' miRNA: 3'- -AUCAGCGCCUGGAGUUUCGGCAUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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