Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1343 | 3' | -58.9 | NC_001335.1 | + | 5577 | 0.66 | 0.502492 |
Target: 5'- aGAUcaGGACGAUGCCCAGcaguGCCGggGCGg -3' miRNA: 3'- -UUGccCUUGCUGCGGGUC----UGGCa-CGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 9709 | 0.66 | 0.502492 |
Target: 5'- cACGcGGGAUGACaCCCuGGCCGuUGCc -3' miRNA: 3'- uUGC-CCUUGCUGcGGGuCUGGC-ACGc -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 21409 | 0.66 | 0.502492 |
Target: 5'- cGAUGGGucggcaaaGACGAuccUGaCCCAGAgcCCGUGCGu -3' miRNA: 3'- -UUGCCC--------UUGCU---GC-GGGUCU--GGCACGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 11375 | 0.66 | 0.533389 |
Target: 5'- cGGCGuGAACGAccccaagguCGCgCAGACCG-GCGa -3' miRNA: 3'- -UUGCcCUUGCU---------GCGgGUCUGGCaCGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 22941 | 0.67 | 0.43376 |
Target: 5'- cGGCGGcGuauCGAgGCCCAGAUCG-GCu -3' miRNA: 3'- -UUGCC-Cuu-GCUgCGGGUCUGGCaCGc -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 24069 | 0.67 | 0.462576 |
Target: 5'- uGGCGGGAACGGCaauGCuCCuGGCgGcgGCGg -3' miRNA: 3'- -UUGCCCUUGCUG---CG-GGuCUGgCa-CGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 50760 | 0.67 | 0.472403 |
Target: 5'- -cCGGGGGCGGCGCaCCuuGGGCgCGUuggcucGCGg -3' miRNA: 3'- uuGCCCUUGCUGCG-GG--UCUG-GCA------CGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 17744 | 0.68 | 0.397016 |
Target: 5'- cGACGGGGAcuCGAUGCCCAGgaaGCCcuucacUGCa -3' miRNA: 3'- -UUGCCCUU--GCUGCGGGUC---UGGc-----ACGc -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 4534 | 0.69 | 0.354001 |
Target: 5'- gAACGGGGAaaaaGAC-CCCAGGCCcgaaagGCGa -3' miRNA: 3'- -UUGCCCUUg---CUGcGGGUCUGGca----CGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 32918 | 0.69 | 0.345802 |
Target: 5'- cGACGGuGACGACgGCCuCGGcuGCUGUGCGc -3' miRNA: 3'- -UUGCCcUUGCUG-CGG-GUC--UGGCACGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 1219 | 0.7 | 0.299501 |
Target: 5'- --aGGGAACGGCaGCgCCGGuACCGgagGCGu -3' miRNA: 3'- uugCCCUUGCUG-CG-GGUC-UGGCa--CGC- -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 41683 | 0.71 | 0.264698 |
Target: 5'- cGugGGGAuCGACGCCUgGGGCCG-GCu -3' miRNA: 3'- -UugCCCUuGCUGCGGG-UCUGGCaCGc -5' |
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1343 | 3' | -58.9 | NC_001335.1 | + | 9083 | 1.07 | 0.000618 |
Target: 5'- cAACGGGAACGACGCCCAGACCGUGCGg -3' miRNA: 3'- -UUGCCCUUGCUGCGGGUCUGGCACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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