Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1343 | 5' | -58.1 | NC_001335.1 | + | 22666 | 0.66 | 0.557602 |
Target: 5'- cACCGC-CGGCCa---CGCCGcCAGCGu -3' miRNA: 3'- -UGGUGaGUCGGgugaGUGGCaGUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 13081 | 0.66 | 0.557602 |
Target: 5'- gGCCAC-C-GCCagaUCACgGUCGGCGg -3' miRNA: 3'- -UGGUGaGuCGGgugAGUGgCAGUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 12771 | 0.66 | 0.557602 |
Target: 5'- gAUCAgaUCGGCCCggaACgUCGCCGacgCAGCGg -3' miRNA: 3'- -UGGUg-AGUCGGG---UG-AGUGGCa--GUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 7220 | 0.66 | 0.556535 |
Target: 5'- gGCCguACUCGGCCUucaGCuucuugcugaucaUCACCGggaaCAGCGa -3' miRNA: 3'- -UGG--UGAGUCGGG---UG-------------AGUGGCa---GUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 3186 | 0.66 | 0.546955 |
Target: 5'- gACCA-UCAGCucccgCCGCUCGCC-UUGGCGa -3' miRNA: 3'- -UGGUgAGUCG-----GGUGAGUGGcAGUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 8739 | 0.66 | 0.546955 |
Target: 5'- cACCACU--GCCacaGCUCuuCGUCAGCc -3' miRNA: 3'- -UGGUGAguCGGg--UGAGugGCAGUCGc -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 23821 | 0.66 | 0.536373 |
Target: 5'- uCCAcCUCuGCCCca--GCCGUCAGCc -3' miRNA: 3'- uGGU-GAGuCGGGugagUGGCAGUCGc -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 41784 | 0.66 | 0.536373 |
Target: 5'- cAUCugUguGCCCGCcagcgUCcCCGUaCAGCGg -3' miRNA: 3'- -UGGugAguCGGGUG-----AGuGGCA-GUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 2397 | 0.66 | 0.536373 |
Target: 5'- aGCCgGCcCAGCUCACUCcuuGCCGaccucgCGGCGa -3' miRNA: 3'- -UGG-UGaGUCGGGUGAG---UGGCa-----GUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 39850 | 0.66 | 0.535318 |
Target: 5'- cGCCGCaUCAGaucgccuCCCACUgcagGCCGUCGuGCGa -3' miRNA: 3'- -UGGUG-AGUC-------GGGUGAg---UGGCAGU-CGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 20525 | 0.66 | 0.515432 |
Target: 5'- cAUCAa-CAGCCaGCUCGCCGcgcUCGGCGc -3' miRNA: 3'- -UGGUgaGUCGGgUGAGUGGC---AGUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 34405 | 0.66 | 0.505085 |
Target: 5'- gGCCAg-CGGCCCGCU-GCCGUUcGCu -3' miRNA: 3'- -UGGUgaGUCGGGUGAgUGGCAGuCGc -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 19537 | 0.67 | 0.494829 |
Target: 5'- cCCACUCGagcaCCCGCUCGCC---AGCGa -3' miRNA: 3'- uGGUGAGUc---GGGUGAGUGGcagUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 38283 | 0.67 | 0.494829 |
Target: 5'- uCCGCUCGGCCgGCcagcccgcgacUCGCCaaauacucGUCGGCu -3' miRNA: 3'- uGGUGAGUCGGgUG-----------AGUGG--------CAGUCGc -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 26598 | 0.67 | 0.464655 |
Target: 5'- aGCCGaaCGGCCUugaGCcgGCCGUCGGCGa -3' miRNA: 3'- -UGGUgaGUCGGG---UGagUGGCAGUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 5782 | 0.67 | 0.454811 |
Target: 5'- cCCAgCUCGGCCCGCUgGCCacgCAGa- -3' miRNA: 3'- uGGU-GAGUCGGGUGAgUGGca-GUCgc -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 46764 | 0.67 | 0.445081 |
Target: 5'- -aCGC-CAGCCuCACggGCgGUCAGCGg -3' miRNA: 3'- ugGUGaGUCGG-GUGagUGgCAGUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 25794 | 0.68 | 0.435468 |
Target: 5'- -gCGgUCGGaaCC-CUCGCCGUCGGCGg -3' miRNA: 3'- ugGUgAGUCg-GGuGAGUGGCAGUCGC- -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 4157 | 0.69 | 0.371723 |
Target: 5'- cGCCGC-CGGCUgcCUCGCCgGUCGGCa -3' miRNA: 3'- -UGGUGaGUCGGguGAGUGG-CAGUCGc -5' |
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1343 | 5' | -58.1 | NC_001335.1 | + | 46268 | 0.69 | 0.354715 |
Target: 5'- cGCCGCguugCGGCCgucgCGCUgACCGUgGGCa -3' miRNA: 3'- -UGGUGa---GUCGG----GUGAgUGGCAgUCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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