miRNA: 3'- cAGUG-CGaC-GGUUGuuACGAGUUCCG- -5'
miRNA: 3'- -CAgUGCGACgGuuGUUACG-AGUUCCG- -5'
miRNA: 3'- cAGUGCGACGGUugUUACGAgu--UCCG- -5'
miRNA: 3'- -CAGUGCGACGGUUGUUACGAGUUCCG- -5'
Results 41 - 44 of 44 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13438 | 5' | -51.5 | NC_003409.1 | + | 27227 | 0.73 | 0.7128 |
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Target: 5'- aUCACuGCgGcCCAAC--UGCUCGAGGCg -3' miRNA: 3'- cAGUG-CGaC-GGUUGuuACGAGUUCCG- -5' |
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| 13438 | 5' | -51.5 | NC_003409.1 | + | 8272 | 0.74 | 0.69168 |
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Target: 5'- uGUgACGCUGgCu-CAGUGCuUCGAGGCu -3' miRNA: 3'- -CAgUGCGACgGuuGUUACG-AGUUCCG- -5' |
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| 13438 | 5' | -51.5 | NC_003409.1 | + | 127471 | 0.79 | 0.422796 |
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Target: 5'- cUUGCGUUGCCAugGAUGCUaccuAGGCa -3' miRNA: 3'- cAGUGCGACGGUugUUACGAgu--UCCG- -5' |
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| 13438 | 5' | -51.5 | NC_003409.1 | + | 109485 | 1.12 | 0.00378 |
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Target: 5'- uGUCACGCUGCCAACAAUGCUCAAGGCu -3' miRNA: 3'- -CAGUGCGACGGUUGUUACGAGUUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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