Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13445 | 5' | -48.6 | NC_003409.1 | + | 45245 | 0.67 | 0.992065 |
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Target: 5'- gGGGCGcCACguCCAGGAcgUGUc-- -3' miRNA: 3'- aCCCGCaGUGguGGUUUUuaACGuuu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 133308 | 0.67 | 0.99498 |
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Target: 5'- aGGGCGUU---GCCAGGAGUggugGCGAu -3' miRNA: 3'- aCCCGCAGuggUGGUUUUUAa---CGUUu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 75164 | 0.67 | 0.99498 |
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Target: 5'- cGGGCG-CGCCACUuugGGAAGgcgcUGUAAGg -3' miRNA: 3'- aCCCGCaGUGGUGG---UUUUUa---ACGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 46967 | 0.66 | 0.995731 |
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Target: 5'- gGGaGCGUacuGCCGCuCGGAGAUUGCc-- -3' miRNA: 3'- aCC-CGCAg--UGGUG-GUUUUUAACGuuu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 79718 | 0.66 | 0.996326 |
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Target: 5'- gUGGcGCGcgcgCACCACCAAAucaggacGcgUGCAc- -3' miRNA: 3'- -ACC-CGCa---GUGGUGGUUU-------UuaACGUuu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 107962 | 0.66 | 0.996851 |
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Target: 5'- gGGGCGUgC-CCACCGuggccugcUGCAAAu -3' miRNA: 3'- aCCCGCA-GuGGUGGUuuuua---ACGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 43659 | 0.66 | 0.997454 |
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Target: 5'- gGGGUgccaaccucGUCACCGCCGuuagcuacGGAAggGCGAu -3' miRNA: 3'- aCCCG---------CAGUGGUGGU--------UUUUaaCGUUu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 38052 | 0.66 | 0.997879 |
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Target: 5'- gGGGCGUCGacuCCGucauguUCAGAAAUaUGCGAGu -3' miRNA: 3'- aCCCGCAGU---GGU------GGUUUUUA-ACGUUU- -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 11169 | 0.66 | 0.997879 |
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Target: 5'- gGGGCucCGCCGCUAGGAggUGCc-- -3' miRNA: 3'- aCCCGcaGUGGUGGUUUUuaACGuuu -5' |
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| 13445 | 5' | -48.6 | NC_003409.1 | + | 87318 | 0.66 | 0.997879 |
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Target: 5'- -cGGCG-CACCACCcaccccAGAGGUgGCAGAg -3' miRNA: 3'- acCCGCaGUGGUGG------UUUUUAaCGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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