Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13447 | 3' | -46.5 | NC_003409.1 | + | 24640 | 0.66 | 0.999887 |
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Target: 5'- cCUGGGGUGcugccggGGCUCCugggguGCUGCcGGg -3' miRNA: 3'- -GGCUCUAC-------UCGAGGuuuu--UGAUGaCC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 24680 | 0.66 | 0.999887 |
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Target: 5'- cCUGGGGUGcugccggGGCUCCugggguGCUGCcGGg -3' miRNA: 3'- -GGCUCUAC-------UCGAGGuuuu--UGAUGaCC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 82428 | 0.66 | 0.999916 |
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Target: 5'- -gGGGGUGGGaUUCCcuGGGCcgACUGGg -3' miRNA: 3'- ggCUCUACUC-GAGGuuUUUGa-UGACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 14359 | 0.66 | 0.999935 |
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Target: 5'- cUCGGGGUGcucugccAGCUCgGAgcgcAGGCUAcCUGGg -3' miRNA: 3'- -GGCUCUAC-------UCGAGgUU----UUUGAU-GACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 14021 | 0.66 | 0.999937 |
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Target: 5'- cCCGcAGuAUGAGgUCCAGGaccuGACUGCUc- -3' miRNA: 3'- -GGC-UC-UACUCgAGGUUU----UUGAUGAcc -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 26367 | 0.66 | 0.999944 |
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Target: 5'- gCCgGAGAUgGAGCgCCAAGAAauuuuugaacuguuuCUGCUGa -3' miRNA: 3'- -GG-CUCUA-CUCGaGGUUUUU---------------GAUGACc -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 71140 | 0.66 | 0.999951 |
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Target: 5'- uCUGGGAUGA-CUgCAAAGAUagauuuaugcugaUACUGGa -3' miRNA: 3'- -GGCUCUACUcGAgGUUUUUG-------------AUGACC- -5' |
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| 13447 | 3' | -46.5 | NC_003409.1 | + | 112456 | 0.66 | 0.999953 |
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Target: 5'- --aAGGUGAGagaccccgugaUCCAGGAGCgACUGGa -3' miRNA: 3'- ggcUCUACUCg----------AGGUUUUUGaUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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