Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1346 | 3' | -56.1 | NC_001335.1 | + | 46008 | 0.66 | 0.678287 |
Target: 5'- gCCGucCCGGUugucguugUCCcGGCGGG-CUUCGCc -3' miRNA: 3'- -GGCu-GGUCG--------AGGuCUGCCCuGAAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 40384 | 0.66 | 0.667431 |
Target: 5'- gCUGAUCAaCUcuccCCAGGCGGGGCgcgCAUg -3' miRNA: 3'- -GGCUGGUcGA----GGUCUGCCCUGaa-GUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 6045 | 0.66 | 0.667431 |
Target: 5'- -gGACgCAGaUCCGuACGcGGACUUCGCg -3' miRNA: 3'- ggCUG-GUCgAGGUcUGC-CCUGAAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 17972 | 0.66 | 0.667431 |
Target: 5'- cUCGACCAGaUCCAGGCGcagaaGGACaUCc- -3' miRNA: 3'- -GGCUGGUCgAGGUCUGC-----CCUGaAGug -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 12796 | 0.66 | 0.656543 |
Target: 5'- aCCuAUCAGCUCCAGGCc-GACcugUCGCa -3' miRNA: 3'- -GGcUGGUCGAGGUCUGccCUGa--AGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 17163 | 0.67 | 0.63471 |
Target: 5'- cUCGGCCAGgUCgCAGGCGcGGuGCUgcagcUCGCu -3' miRNA: 3'- -GGCUGGUCgAG-GUCUGC-CC-UGA-----AGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 17213 | 0.67 | 0.612867 |
Target: 5'- gCCGucGCCAGCgCCGGgauguGCGGGGCcagacuggUCACc -3' miRNA: 3'- -GGC--UGGUCGaGGUC-----UGCCCUGa-------AGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 20252 | 0.67 | 0.612867 |
Target: 5'- aCUGGCCGGCgacggCCAGGuCGaGGGCgUCGa -3' miRNA: 3'- -GGCUGGUCGa----GGUCU-GC-CCUGaAGUg -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 36712 | 0.67 | 0.601966 |
Target: 5'- cUCGuuCCAGUUCCAGAaccugcuGGACUUCGa -3' miRNA: 3'- -GGCu-GGUCGAGGUCUgc-----CCUGAAGUg -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 31069 | 0.67 | 0.59109 |
Target: 5'- uUCGGCCGGCUCgGGcCGGucucaGGCUUgACc -3' miRNA: 3'- -GGCUGGUCGAGgUCuGCC-----CUGAAgUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 12188 | 0.67 | 0.580248 |
Target: 5'- gCCGACCuGGCcCCAGACGagcuGGCggaagUCACc -3' miRNA: 3'- -GGCUGG-UCGaGGUCUGCc---CUGa----AGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 15431 | 0.68 | 0.569449 |
Target: 5'- gCGACCuGUUCCGcGACGaGGAUccgcuUUCGCc -3' miRNA: 3'- gGCUGGuCGAGGU-CUGC-CCUG-----AAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 47308 | 0.68 | 0.557628 |
Target: 5'- gUCGACCAGCgagcguUCCcaugcgaggaGGACGaagucuuGGGCUUCGCu -3' miRNA: 3'- -GGCUGGUCG------AGG----------UCUGC-------CCUGAAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 23171 | 0.68 | 0.548009 |
Target: 5'- uCCGACCAuGUggCCAGcACGGGGCcgUUCu- -3' miRNA: 3'- -GGCUGGU-CGa-GGUC-UGCCCUG--AAGug -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 24029 | 0.68 | 0.537383 |
Target: 5'- aCGACCAGCUCUacauAGGCGGcg---CACa -3' miRNA: 3'- gGCUGGUCGAGG----UCUGCCcugaaGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 6610 | 0.68 | 0.516353 |
Target: 5'- gCGGCCAGCUCgGGccuUGGGucgACUUgGCg -3' miRNA: 3'- gGCUGGUCGAGgUCu--GCCC---UGAAgUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 7932 | 0.69 | 0.495663 |
Target: 5'- gCCGACgAGCgcggUCCAGuCGuuGGACUUCGa -3' miRNA: 3'- -GGCUGgUCG----AGGUCuGC--CCUGAAGUg -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 4700 | 0.69 | 0.465359 |
Target: 5'- uCUGGCCugguGCUgCAGAcCGGGGCUugguauggaggUCGCa -3' miRNA: 3'- -GGCUGGu---CGAgGUCU-GCCCUGA-----------AGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 38764 | 0.7 | 0.455473 |
Target: 5'- cUCGACgCAGCggagaCCGG-CGaGGGCUUCGCc -3' miRNA: 3'- -GGCUG-GUCGa----GGUCuGC-CCUGAAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 43212 | 0.7 | 0.445701 |
Target: 5'- aUGACCuaugacguggaGGCUCCgaAGACGGGcgacuACUUCAUg -3' miRNA: 3'- gGCUGG-----------UCGAGG--UCUGCCC-----UGAAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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