miRNA display CGI


Results 21 - 24 of 24 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1346 3' -56.1 NC_001335.1 + 45548 0.72 0.314725
Target:  5'- aCGGUCAGCgaCCAGACGGuGAuCUUCACu -3'
miRNA:   3'- gGCUGGUCGa-GGUCUGCC-CU-GAAGUG- -5'
1346 3' -56.1 NC_001335.1 + 47707 0.73 0.292288
Target:  5'- aCCGAggcUCGGCUCCAGA-GGGACcacaucaucgUCACg -3'
miRNA:   3'- -GGCU---GGUCGAGGUCUgCCCUGa---------AGUG- -5'
1346 3' -56.1 NC_001335.1 + 13905 0.76 0.183363
Target:  5'- aCGACaugGGUUCCAGACGuGGACUUC-Cg -3'
miRNA:   3'- gGCUGg--UCGAGGUCUGC-CCUGAAGuG- -5'
1346 3' -56.1 NC_001335.1 + 11230 1.11 0.000618
Target:  5'- gCCGACCAGCUCCAGACGGGACUUCACu -3'
miRNA:   3'- -GGCUGGUCGAGGUCUGCCCUGAAGUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.