Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1346 | 3' | -56.1 | NC_001335.1 | + | 7932 | 0.69 | 0.495663 |
Target: 5'- gCCGACgAGCgcggUCCAGuCGuuGGACUUCGa -3' miRNA: 3'- -GGCUGgUCG----AGGUCuGC--CCUGAAGUg -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 6610 | 0.68 | 0.516353 |
Target: 5'- gCGGCCAGCUCgGGccuUGGGucgACUUgGCg -3' miRNA: 3'- gGCUGGUCGAGgUCu--GCCC---UGAAgUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 6045 | 0.66 | 0.667431 |
Target: 5'- -gGACgCAGaUCCGuACGcGGACUUCGCg -3' miRNA: 3'- ggCUG-GUCgAGGUcUGC-CCUGAAGUG- -5' |
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1346 | 3' | -56.1 | NC_001335.1 | + | 4700 | 0.69 | 0.465359 |
Target: 5'- uCUGGCCugguGCUgCAGAcCGGGGCUugguauggaggUCGCa -3' miRNA: 3'- -GGCUGGu---CGAgGUCU-GCCCUGA-----------AGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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