miRNA: 3'- aCAGUGUA---AGACAC-UGG---CGCU- -5'
miRNA: 3'- acAGUGUaAGACa---CUGGCGCU- -5'
miRNA: 3'- -ACAGUguaa-GACA-CUGGCGCU- -5'
miRNA: 3'- aCAGUGUAAGaca-CUGGCgCU- -5'
miRNA: 3'- aCA-GUGUAAGACACUGGcgcu -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13460 | 3' | -42.2 | NC_003409.1 | + | 25656 | 0.68 | 0.999995 |
|
Target: 5'- cGUCACAUaucUCUGUGcACCcaaGUGGu -3' miRNA: 3'- aCAGUGUA---AGACAC-UGG---CGCU- -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 15486 | 0.7 | 0.999821 |
|
Target: 5'- -cUCACAgUCUGgauaGACUGCGAa -3' miRNA: 3'- acAGUGUaAGACa---CUGGCGCU- -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 9214 | 0.67 | 0.999999 |
|
Target: 5'- cUGUCAagcccCUGUaGACCGUGAc -3' miRNA: 3'- -ACAGUguaa-GACA-CUGGCGCU- -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 8415 | 0.68 | 0.999995 |
|
Target: 5'- cGUCACAUUUggagGACUGgGAa -3' miRNA: 3'- aCAGUGUAAGaca-CUGGCgCU- -5' |
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| 13460 | 3' | -42.2 | NC_003409.1 | + | 5264 | 0.67 | 0.999998 |
|
Target: 5'- aGUcCAUGUUCUGUGACCu--- -3' miRNA: 3'- aCA-GUGUAAGACACUGGcgcu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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