Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13468 | 5' | -59.3 | NC_003409.1 | + | 131509 | 0.72 | 0.401508 |
Target: 5'- -gGGGCCAacggUGCCcAGuGGGUGUGCCa -3' miRNA: 3'- caUCCGGU----ACGGaUCuCCCGCGUGGc -5' |
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13468 | 5' | -59.3 | NC_003409.1 | + | 25024 | 0.72 | 0.38472 |
Target: 5'- -cAGGUUGuUGCCggggAGGGuGGCGCGCCGg -3' miRNA: 3'- caUCCGGU-ACGGa---UCUC-CCGCGUGGC- -5' |
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13468 | 5' | -59.3 | NC_003409.1 | + | 50752 | 0.73 | 0.344833 |
Target: 5'- gGUGGGCacggaGUGCUUugcGAGGGUGUGCCGc -3' miRNA: 3'- -CAUCCGg----UACGGAu--CUCCCGCGUGGC- -5' |
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13468 | 5' | -59.3 | NC_003409.1 | + | 49274 | 0.75 | 0.255467 |
Target: 5'- --uGGCCcUGCCUaguGGGGuGGCGCGCCa -3' miRNA: 3'- cauCCGGuACGGA---UCUC-CCGCGUGGc -5' |
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13468 | 5' | -59.3 | NC_003409.1 | + | 134298 | 1.08 | 0.001367 |
Target: 5'- cGUAGGCCAUGCCUAGAGGGCGCACCGc -3' miRNA: 3'- -CAUCCGGUACGGAUCUCCCGCGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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