Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1347 | 5' | -57.9 | NC_001335.1 | + | 37947 | 0.66 | 0.556776 |
Target: 5'- gGAGuUCGCCCggucGGUGGCgaaGACGcuGCCGa -3' miRNA: 3'- -UUC-AGUGGGa---CCAUCGg--CUGC--UGGCa -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 43701 | 0.66 | 0.550355 |
Target: 5'- -cGUCGCUgaGGUcuacgagaaggcucuGGCCGAgGGCCGc -3' miRNA: 3'- uuCAGUGGgaCCA---------------UCGGCUgCUGGCa -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 5619 | 0.67 | 0.50407 |
Target: 5'- -uGUCACCgaGGUcAGCCGcCGACUc- -3' miRNA: 3'- uuCAGUGGgaCCA-UCGGCuGCUGGca -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 22857 | 0.67 | 0.463513 |
Target: 5'- -cGUCAUcuUCUGGUAGUCGGUGGCUGUc -3' miRNA: 3'- uuCAGUG--GGACCAUCGGCUGCUGGCA- -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 16000 | 0.67 | 0.46252 |
Target: 5'- gAAGUCgGCCUUGGccugGGCCgcaucgaGAUGACCGUu -3' miRNA: 3'- -UUCAG-UGGGACCa---UCGG-------CUGCUGGCA- -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 20995 | 0.69 | 0.370362 |
Target: 5'- uGGU-GCUCgaccgGGUAGCCGAgGACCGa -3' miRNA: 3'- uUCAgUGGGa----CCAUCGGCUgCUGGCa -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 38246 | 0.69 | 0.344196 |
Target: 5'- uGGUCGCCCUggcgucacuggacGGUAGCUG-CGAuCCGg -3' miRNA: 3'- uUCAGUGGGA-------------CCAUCGGCuGCU-GGCa -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 28013 | 0.74 | 0.182321 |
Target: 5'- gGAGUUGCCCUGGUAcaucGCUGAgGACgGUa -3' miRNA: 3'- -UUCAGUGGGACCAU----CGGCUgCUGgCA- -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 12161 | 1.05 | 0.000874 |
Target: 5'- gAAGUCACCCUGGUAGCCGACGACCGUg -3' miRNA: 3'- -UUCAGUGGGACCAUCGGCUGCUGGCA- -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 25891 | 0.72 | 0.231268 |
Target: 5'- uGGU-GCCCuUGGUGGUCGACGGCCu- -3' miRNA: 3'- uUCAgUGGG-ACCAUCGGCUGCUGGca -5' |
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1347 | 5' | -57.9 | NC_001335.1 | + | 22333 | 0.66 | 0.546088 |
Target: 5'- --aUCACCCcggagGGggAGCUGAuCGACCGg -3' miRNA: 3'- uucAGUGGGa----CCa-UCGGCU-GCUGGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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