Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1351 | 3' | -57.1 | NC_001335.1 | + | 33803 | 0.66 | 0.597361 |
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Target: 5'- gUCGAGGuagucggucuugccGGCcggCGUCAcGAaCGCCGCCa -3' miRNA: 3'- -AGCUCCu-------------CCGa--GUAGU-CUcGUGGCGG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 35034 | 0.66 | 0.582175 |
|
Target: 5'- cUUGAaauAGGCgUCggCAGAGuCGCCGCCa -3' miRNA: 3'- -AGCUcc-UCCG-AGuaGUCUC-GUGGCGG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 17376 | 0.66 | 0.582175 |
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Target: 5'- aUCGGcGGcGcGCUCAUCGGcGCGCUGgCg -3' miRNA: 3'- -AGCU-CCuC-CGAGUAGUCuCGUGGCgG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 3137 | 0.66 | 0.582175 |
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Target: 5'- cCGAGGGGuuUC--UAGAGCcGCUGCCg -3' miRNA: 3'- aGCUCCUCcgAGuaGUCUCG-UGGCGG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 43733 | 0.66 | 0.625697 |
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Target: 5'- gCGuGGuuGC-CG-CAGGGCACCGCg -3' miRNA: 3'- aGCuCCucCGaGUaGUCUCGUGGCGg -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 47554 | 0.66 | 0.571375 |
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Target: 5'- -gGAGGuaaGGGCgguggccaCAUCGGAGCugUgGCCa -3' miRNA: 3'- agCUCC---UCCGa-------GUAGUCUCGugG-CGG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 1512 | 0.66 | 0.625697 |
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Target: 5'- cCGAGGAGGagaUCGUC----CACCGCa -3' miRNA: 3'- aGCUCCUCCg--AGUAGucucGUGGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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