Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1351 | 3' | -57.1 | NC_001335.1 | + | 2698 | 0.67 | 0.560624 |
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Target: 5'- -aGAGcGGGUUCGUCGGAaCACC-CCg -3' miRNA: 3'- agCUCcUCCGAGUAGUCUcGUGGcGG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 24726 | 0.67 | 0.548863 |
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Target: 5'- cCGAaccGGAGGCUCGUCcacgccaGGAuGUauGCCGCg -3' miRNA: 3'- aGCU---CCUCCGAGUAG-------UCU-CG--UGGCGg -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 30147 | 0.69 | 0.397687 |
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Target: 5'- gUCGaAGGAGGCgu-UCuGGAGCACCaugcgcuugagagcGCCg -3' miRNA: 3'- -AGC-UCCUCCGaguAG-UCUCGUGG--------------CGG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 1865 | 0.7 | 0.356589 |
|
Target: 5'- aUCGAGG-GGCUgGUCGauccGAGCAagacCCGCg -3' miRNA: 3'- -AGCUCCuCCGAgUAGU----CUCGU----GGCGg -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 28443 | 0.71 | 0.337593 |
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Target: 5'- cCGAGGAccgugaacuuccucGGCUcCAUCAGGGCAC-GUCc -3' miRNA: 3'- aGCUCCU--------------CCGA-GUAGUCUCGUGgCGG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 28853 | 0.71 | 0.316247 |
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Target: 5'- cUCGuGGAGGCgaAUCAGAGC-CCGaCa -3' miRNA: 3'- -AGCuCCUCCGagUAGUCUCGuGGCgG- -5' |
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| 1351 | 3' | -57.1 | NC_001335.1 | + | 1512 | 0.66 | 0.625697 |
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Target: 5'- cCGAGGAGGagaUCGUC----CACCGCa -3' miRNA: 3'- aGCUCCUCCg--AGUAGucucGUGGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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