Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13613 | 3' | -59.5 | NC_003460.1 | + | 1323 | 0.66 | 0.08053 |
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Target: 5'- aGCAGaaaCAGUgCCACCGACaucgcuCGGaGCCu -3' miRNA: 3'- gUGUCa--GUCAgGGUGGCUG------GCCgCGG- -5' |
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| 13613 | 3' | -59.5 | NC_003460.1 | + | 523 | 0.66 | 0.075394 |
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Target: 5'- aACAG-CcGUCCCGCCcaccaGACCGGU-CCa -3' miRNA: 3'- gUGUCaGuCAGGGUGG-----CUGGCCGcGG- -5' |
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| 13613 | 3' | -59.5 | NC_003460.1 | + | 3857 | 0.67 | 0.063891 |
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Target: 5'- cCGCAGUCuG-CCCGCCcGCCGaaagaGCCg -3' miRNA: 3'- -GUGUCAGuCaGGGUGGcUGGCcg---CGG- -5' |
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| 13613 | 3' | -59.5 | NC_003460.1 | + | 803 | 0.68 | 0.048931 |
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Target: 5'- --uGGUCAGUUCgucuCCGAcuCCGGUGCCu -3' miRNA: 3'- gugUCAGUCAGGgu--GGCU--GGCCGCGG- -5' |
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| 13613 | 3' | -59.5 | NC_003460.1 | + | 927 | 0.7 | 0.034965 |
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Target: 5'- aACAc-CAGcaaCCCGCCGACCGGCuCCg -3' miRNA: 3'- gUGUcaGUCa--GGGUGGCUGGCCGcGG- -5' |
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| 13613 | 3' | -59.5 | NC_003460.1 | + | 5590 | 0.75 | 0.013519 |
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Target: 5'- uCACAG-CAGUCCacggaGCCGGCUGGgGCa -3' miRNA: 3'- -GUGUCaGUCAGGg----UGGCUGGCCgCGg -5' |
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| 13613 | 3' | -59.5 | NC_003460.1 | + | 2166 | 1.11 | 8e-06 |
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Target: 5'- gCACAGUCAGUCCCACCGACCGGCGCCg -3' miRNA: 3'- -GUGUCAGUCAGGGUGGCUGGCCGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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