Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1363 | 3' | -55 | NC_001335.1 | + | 6153 | 0.7 | 0.466784 |
Target: 5'- aCGAUG-GCUCCCug-CGAGUAgCCGg -3' miRNA: 3'- aGCUACuUGAGGGugaGCUCGUgGGC- -5' |
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1363 | 3' | -55 | NC_001335.1 | + | 6187 | 0.71 | 0.446955 |
Target: 5'- gUUGAUGAugUggUGCUUGAGCACCUGg -3' miRNA: 3'- -AGCUACUugAggGUGAGCUCGUGGGC- -5' |
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1363 | 3' | -55 | NC_001335.1 | + | 19548 | 1.08 | 0.001212 |
Target: 5'- cUCGAUGAACUCCCACUCGAGCACCCGc -3' miRNA: 3'- -AGCUACUUGAGGGUGAGCUCGUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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