Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1363 | 3' | -55 | NC_001335.1 | + | 48865 | 0.66 | 0.745526 |
Target: 5'- aCGAUGAGCUUgaaCACUUGGuGCcuACCCu -3' miRNA: 3'- aGCUACUUGAGg--GUGAGCU-CG--UGGGc -5' |
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1363 | 3' | -55 | NC_001335.1 | + | 22410 | 0.65 | 0.755883 |
Target: 5'- gUCGAUcAGCUCCCcCUCcgGGGUgauGCCCa -3' miRNA: 3'- -AGCUAcUUGAGGGuGAG--CUCG---UGGGc -5' |
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1363 | 3' | -55 | NC_001335.1 | + | 13111 | 0.65 | 0.755883 |
Target: 5'- cUGAUGAGC-CUC-CUCGAcaccgGUGCCCGg -3' miRNA: 3'- aGCUACUUGaGGGuGAGCU-----CGUGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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