Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1364 | 5' | -55.6 | NC_001335.1 | + | 11590 | 0.67 | 0.611644 |
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Target: 5'- uGCGgGAUCuUCUGG-CCGGUcGUGCCc -3' miRNA: 3'- cUGCgCUAG-AGGCUaGGUCGuCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 32891 | 0.67 | 0.588593 |
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Target: 5'- uGCGCGA-CUCgauggCGGUCCAGCAcuccaccgaugggGUGUCg -3' miRNA: 3'- cUGCGCUaGAG-----GCUAGGUCGU-------------CACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 35477 | 0.68 | 0.578759 |
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Target: 5'- -cCGcCGAUCUCCGAguUCUugGGagggaGGUGCCa -3' miRNA: 3'- cuGC-GCUAGAGGCU--AGG--UCg----UCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 44446 | 0.68 | 0.54627 |
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Target: 5'- gGAUGaCGAUCUCCuGAUCCGGguGaacgaGCa -3' miRNA: 3'- -CUGC-GCUAGAGG-CUAGGUCguCa----CGg -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 9888 | 0.68 | 0.545197 |
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Target: 5'- cGACuCGAUggCCGGcuguggcUUCGGCGGUGCCg -3' miRNA: 3'- -CUGcGCUAgaGGCU-------AGGUCGUCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 5584 | 0.69 | 0.524935 |
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Target: 5'- -cCGCcccaGAUCaggaCGAUgcCCAGCAGUGCCg -3' miRNA: 3'- cuGCG----CUAGag--GCUA--GGUCGUCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 16151 | 0.69 | 0.514386 |
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Target: 5'- cGGCGUcaucgccuaGAUCUCCGAgcccCCGGC--UGCCg -3' miRNA: 3'- -CUGCG---------CUAGAGGCUa---GGUCGucACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 1823 | 0.69 | 0.493555 |
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Target: 5'- uGACGCGGUCcuugaCCGAgUCCuucauguagAGCuuGGUGCCg -3' miRNA: 3'- -CUGCGCUAGa----GGCU-AGG---------UCG--UCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 19202 | 0.66 | 0.666676 |
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Target: 5'- --aGCGAUCUgcuccUCGcgCCGGuCGGUGUCg -3' miRNA: 3'- cugCGCUAGA-----GGCuaGGUC-GUCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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