Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1364 | 5' | -55.6 | NC_001335.1 | + | 32872 | 0.66 | 0.699387 |
Target: 5'- cGGCGCGGUCUCaGGcUCAGgAGccgggGCCg -3' miRNA: 3'- -CUGCGCUAGAGgCUaGGUCgUCa----CGG- -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 32891 | 0.67 | 0.588593 |
Target: 5'- uGCGCGA-CUCgauggCGGUCCAGCAcuccaccgaugggGUGUCg -3' miRNA: 3'- cUGCGCUaGAG-----GCUAGGUCGU-------------CACGG- -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 34459 | 0.67 | 0.644688 |
Target: 5'- cGACGUGAg--CCGcAUCCGGCcgcaAGggGCCa -3' miRNA: 3'- -CUGCGCUagaGGC-UAGGUCG----UCa-CGG- -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 35477 | 0.68 | 0.578759 |
Target: 5'- -cCGcCGAUCUCCGAguUCUugGGagggaGGUGCCa -3' miRNA: 3'- cuGC-GCUAGAGGCU--AGG--UCg----UCACGG- -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 40503 | 0.66 | 0.710174 |
Target: 5'- cGACGCGGUgUCCGcguugAUUgAGCAccgGCCc -3' miRNA: 3'- -CUGCGCUAgAGGC-----UAGgUCGUca-CGG- -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 40654 | 0.69 | 0.473119 |
Target: 5'- cACGCGGagaggcccaggaUCgCCGAUCCGGCgaugAGUGCg -3' miRNA: 3'- cUGCGCU------------AGaGGCUAGGUCG----UCACGg -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 44446 | 0.68 | 0.54627 |
Target: 5'- gGAUGaCGAUCUCCuGAUCCGGguGaacgaGCa -3' miRNA: 3'- -CUGC-GCUAGAGG-CUAGGUCguCa----CGg -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 46493 | 0.66 | 0.655692 |
Target: 5'- cGACcgGCGGUagCUCuUGAgcuggCCAGCGGUGCUc -3' miRNA: 3'- -CUG--CGCUA--GAG-GCUa----GGUCGUCACGG- -5' |
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1364 | 5' | -55.6 | NC_001335.1 | + | 50778 | 0.7 | 0.453118 |
Target: 5'- gGGCGCGuuggCUCgCGGUgCCuuacggauGGCGGUGCCu -3' miRNA: 3'- -CUGCGCua--GAG-GCUA-GG--------UCGUCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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