Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1369 | 3' | -55.8 | NC_001335.1 | + | 37965 | 0.66 | 0.635077 |
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Target: 5'- --cGGCGacgACGCCGguauGGAguUCGcCCGGUCg -3' miRNA: 3'- caaCCGU---UGCGGU----UCU--GGCaGGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 38350 | 0.69 | 0.453781 |
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Target: 5'- -cUGGCcg-GCCGAGcggaacuaaGCCGUUCGGUCu -3' miRNA: 3'- caACCGuugCGGUUC---------UGGCAGGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 39787 | 0.68 | 0.558151 |
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Target: 5'- -gUGGcCAACGCUucccAGACCGUggugCGGUCc -3' miRNA: 3'- caACC-GUUGCGGu---UCUGGCAg---GCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 41682 | 0.69 | 0.463768 |
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Target: 5'- -gUGGggauCGACGCCuGGGGCCGgCUGGUCu -3' miRNA: 3'- caACC----GUUGCGG-UUCUGGCaGGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 42428 | 0.66 | 0.668191 |
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Target: 5'- cUUGG--ACGCCGAGACgcacgcggcuCGcgCCGGUCg -3' miRNA: 3'- cAACCguUGCGGUUCUG----------GCa-GGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 48382 | 0.66 | 0.668191 |
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Target: 5'- -cUGGCccACGCCAAGggugugguucgGCCGgCCGGcCg -3' miRNA: 3'- caACCGu-UGCGGUUC-----------UGGCaGGCCaG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 49536 | 0.66 | 0.635076 |
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Target: 5'- cUUGGUAA--CCAAGACCaaagGUCuCGGUCa -3' miRNA: 3'- cAACCGUUgcGGUUCUGG----CAG-GCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 51824 | 0.67 | 0.624019 |
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Target: 5'- --cGGCAcggGCuGCCGGGACUGUCCa--- -3' miRNA: 3'- caaCCGU---UG-CGGUUCUGGCAGGccag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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