Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1369 | 3' | -55.8 | NC_001335.1 | + | 26672 | 0.67 | 0.612969 |
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Target: 5'- --cGGCcg-GCuCAAGGCCGUUCGGcUCg -3' miRNA: 3'- caaCCGuugCG-GUUCUGGCAGGCC-AG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 4248 | 0.67 | 0.612969 |
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Target: 5'- --aGGgGACgGCCcgAAGGCCGUCUGGcCc -3' miRNA: 3'- caaCCgUUG-CGG--UUCUGGCAGGCCaG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 16372 | 0.67 | 0.598631 |
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Target: 5'- --gGGCAGCGUCGAGgcggcugccuugauGCCGUCCa--- -3' miRNA: 3'- caaCCGUUGCGGUUC--------------UGGCAGGccag -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 8600 | 0.68 | 0.558151 |
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Target: 5'- --cGGUGACGCCGAuGGCC-UCUGGcCg -3' miRNA: 3'- caaCCGUUGCGGUU-CUGGcAGGCCaG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 24415 | 0.68 | 0.525909 |
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Target: 5'- --gGuGCGACGaCCAAGGagcugauggcCCGUCuCGGUCa -3' miRNA: 3'- caaC-CGUUGC-GGUUCU----------GGCAG-GCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 11895 | 0.69 | 0.473869 |
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Target: 5'- cGUUGGa---GCCGGuGCCGUCCGGg- -3' miRNA: 3'- -CAACCguugCGGUUcUGGCAGGCCag -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 41682 | 0.69 | 0.463768 |
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Target: 5'- -gUGGggauCGACGCCuGGGGCCGgCUGGUCu -3' miRNA: 3'- caACC----GUUGCGG-UUCUGGCaGGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 42428 | 0.66 | 0.668191 |
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Target: 5'- cUUGG--ACGCCGAGACgcacgcggcuCGcgCCGGUCg -3' miRNA: 3'- cAACCguUGCGGUUCUG----------GCa-GGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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