Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1370 | 5' | -61.5 | NC_001335.1 | + | 32424 | 0.66 | 0.356727 |
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Target: 5'- aGGGGAGCuguagaaggGCUUcGCUGCCCcCUGGCu -3' miRNA: 3'- aUCCUUCG---------UGGGcUGACGGGcGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 32984 | 0.66 | 0.347741 |
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Target: 5'- -cGuGGAGCACCgCccCUGCCCagcccgaGCCGGCc -3' miRNA: 3'- auC-CUUCGUGG-GcuGACGGG-------CGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 12720 | 0.67 | 0.301607 |
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Target: 5'- gGGGAuccGGCGCuuGAucaugcgcucgauCUGCUggaGCCGGCg -3' miRNA: 3'- aUCCU---UCGUGggCU-------------GACGGg--CGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 31407 | 0.68 | 0.288016 |
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Target: 5'- gAGGAagaAGCACCUGAaaGCUgCGCUGGg -3' miRNA: 3'- aUCCU---UCGUGGGCUgaCGG-GCGGCCg -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 4176 | 0.68 | 0.28037 |
|
Target: 5'- -cGGccGAGCugCuuugcaucgccgcCGGCUGCCuCGCCGGUc -3' miRNA: 3'- auCC--UUCGugG-------------GCUGACGG-GCGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 6022 | 0.66 | 0.390758 |
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Target: 5'- cGGGuAGUugCCGauggGCUGCCa-CCGGUa -3' miRNA: 3'- aUCCuUCGugGGC----UGACGGgcGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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