Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1370 | 5' | -61.5 | NC_001335.1 | + | 32009 | 0.66 | 0.37263 |
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Target: 5'- -uGGAuccgaccgaccuuGGCAUCCauCUGCCC-CCGGCu -3' miRNA: 3'- auCCU-------------UCGUGGGcuGACGGGcGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 7593 | 0.66 | 0.37348 |
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Target: 5'- cGGGggGaucucggagACCgGGgUGUCgGCCGGCg -3' miRNA: 3'- aUCCuuCg--------UGGgCUgACGGgCGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 39607 | 0.66 | 0.376894 |
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Target: 5'- cAGucGGCACCCGGCacugcaugcgagagGCCgaaaggGCCGGCa -3' miRNA: 3'- aUCcuUCGUGGGCUGa-------------CGGg-----CGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 2366 | 0.66 | 0.382054 |
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Target: 5'- cGGcGAAGUucaGCCuCGACcucgcugGCCCGCUGGg -3' miRNA: 3'- aUC-CUUCG---UGG-GCUGa------CGGGCGGCCg -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 8605 | 0.66 | 0.390758 |
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Target: 5'- cGGGAcGGUgACgCCGAUgGCCUcugGCCGGCg -3' miRNA: 3'- aUCCU-UCG-UG-GGCUGaCGGG---CGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 6022 | 0.66 | 0.390758 |
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Target: 5'- cGGGuAGUugCCGauggGCUGCCa-CCGGUa -3' miRNA: 3'- aUCCuUCGugGGC----UGACGGgcGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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