Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1370 | 5' | -61.5 | NC_001335.1 | + | 38899 | 0.73 | 0.132488 |
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Target: 5'- cGGGccaucGAGUACCUG-CUGaaCCCGCCGGCc -3' miRNA: 3'- aUCC-----UUCGUGGGCuGAC--GGGCGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 30018 | 0.73 | 0.118858 |
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Target: 5'- gAGcGAGUGCCCGAa--CCCGCCGGCc -3' miRNA: 3'- aUCcUUCGUGGGCUgacGGGCGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 41763 | 0.75 | 0.087588 |
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Target: 5'- aUGGGAAGUggcucagcagaacAUCUGugUGCCCGCCaGCg -3' miRNA: 3'- -AUCCUUCG-------------UGGGCugACGGGCGGcCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 32631 | 0.76 | 0.075156 |
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Target: 5'- cAGGAAGCGCCUGguggcgagaagcgguACUGCCaGCaCGGCg -3' miRNA: 3'- aUCCUUCGUGGGC---------------UGACGGgCG-GCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 1366 | 0.77 | 0.064607 |
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Target: 5'- gUGGGggGCACCgGgguguuggggcuGCUGCcaccgCCGCCGGCu -3' miRNA: 3'- -AUCCuuCGUGGgC------------UGACG-----GGCGGCCG- -5' |
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| 1370 | 5' | -61.5 | NC_001335.1 | + | 25011 | 1.1 | 0.000189 |
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Target: 5'- gUAGGAAGCACCCGACUGCCCGCCGGCa -3' miRNA: 3'- -AUCCUUCGUGGGCUGACGGGCGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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