Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13958 | 5' | -51 | NC_003521.1 | + | 120906 | 0.67 | 0.994207 |
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Target: 5'- cGggGGCGGCUGCuugcGUUUgcgGCUGCc- -3' miRNA: 3'- -CuuCUGCCGAUGca--CAAGa--CGAUGca -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 132635 | 0.67 | 0.994207 |
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Target: 5'- cGgcGGCGGCgccaccgGCG-GUUCUGCUuccuCGUc -3' miRNA: 3'- -CuuCUGCCGa------UGCaCAAGACGAu---GCA- -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 239557 | 0.67 | 0.995632 |
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Target: 5'- -cGGGCGGCUagggucaGCGUGUUgcGCUGCa- -3' miRNA: 3'- cuUCUGCCGA-------UGCACAAgaCGAUGca -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 129944 | 0.67 | 0.995698 |
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Target: 5'- cGAGACGGCgca--GUUCUGCcGCGg -3' miRNA: 3'- cUUCUGCCGaugcaCAAGACGaUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 129908 | 0.66 | 0.998108 |
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Target: 5'- cGGGGCGGCcGCGcgc-CUGCUGCGc -3' miRNA: 3'- cUUCUGCCGaUGCacaaGACGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 148794 | 0.66 | 0.998417 |
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Target: 5'- aGGAGccGCGGCUGCGgacGUUCaucgUGCgcgGCGa -3' miRNA: 3'- -CUUC--UGCCGAUGCa--CAAG----ACGa--UGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 105927 | 0.66 | 0.998417 |
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Target: 5'- -uAGACGGuCUGCGUGUg--GCcgGCGc -3' miRNA: 3'- cuUCUGCC-GAUGCACAagaCGa-UGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 135770 | 0.66 | 0.998683 |
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Target: 5'- cGAAGGCGGUgGCGgcaGUggcGCUGCGg -3' miRNA: 3'- -CUUCUGCCGaUGCa--CAagaCGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 58033 | 0.72 | 0.937775 |
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Target: 5'- cGAGGGCGcGC-GCGUGUUgCUGCUgGCGg -3' miRNA: 3'- -CUUCUGC-CGaUGCACAA-GACGA-UGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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