Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13958 | 5' | -51 | NC_003521.1 | + | 138740 | 0.69 | 0.986644 |
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Target: 5'- -cGGAgGGUUACGUGcUcuauuucuacauccUCUGCUACGa -3' miRNA: 3'- cuUCUgCCGAUGCAC-A--------------AGACGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 42277 | 0.69 | 0.985646 |
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Target: 5'- --cGGCGGCgucugucgugccgcCGUGUUgCUGCUGCGa -3' miRNA: 3'- cuuCUGCCGau------------GCACAA-GACGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 174031 | 0.69 | 0.985474 |
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Target: 5'- aGGAGACGGCgguggcccgcACGUGg---GCUGCGa -3' miRNA: 3'- -CUUCUGCCGa---------UGCACaagaCGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 133335 | 0.69 | 0.979552 |
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Target: 5'- --uGGCGGCUGCGauggcUGUgacugCUGCUGCu- -3' miRNA: 3'- cuuCUGCCGAUGC-----ACAa----GACGAUGca -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 73911 | 0.71 | 0.955272 |
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Target: 5'- uGGAGGCGGCcgcgGCGgagGccUUCUGCUugGg -3' miRNA: 3'- -CUUCUGCCGa---UGCa--C--AAGACGAugCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 195974 | 0.71 | 0.951252 |
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Target: 5'- uGGAGGCGuuuuacgacacGCUGCGgGcgCUGCUACGUa -3' miRNA: 3'- -CUUCUGC-----------CGAUGCaCaaGACGAUGCA- -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 58033 | 0.72 | 0.937775 |
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Target: 5'- cGAGGGCGcGC-GCGUGUUgCUGCUgGCGg -3' miRNA: 3'- -CUUCUGC-CGaUGCACAA-GACGA-UGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 53424 | 0.81 | 0.494364 |
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Target: 5'- cGAGGGCGGCcGCGUGUaCUGUUACGc -3' miRNA: 3'- -CUUCUGCCGaUGCACAaGACGAUGCa -5' |
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| 13958 | 5' | -51 | NC_003521.1 | + | 27448 | 1.09 | 0.013659 |
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Target: 5'- cGAAGACGGCUACGUGUUCUGCUACGUg -3' miRNA: 3'- -CUUCUGCCGAUGCACAAGACGAUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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