Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13960 | 5' | -57.2 | NC_003521.1 | + | 137130 | 0.69 | 0.780098 |
Target: 5'- --aGAUGcGCUUGUCGGgacAGCGCAGGc -3' miRNA: 3'- ccaCUGCcCGAACAGCC---UCGCGUCUu -5' |
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13960 | 5' | -57.2 | NC_003521.1 | + | 166182 | 0.69 | 0.823257 |
Target: 5'- cGGUGGCGGcGgUggaGUCGGcccggcAGCGCGGGGu -3' miRNA: 3'- -CCACUGCC-CgAa--CAGCC------UCGCGUCUU- -5' |
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13960 | 5' | -57.2 | NC_003521.1 | + | 126292 | 0.68 | 0.869611 |
Target: 5'- -cUGACGuucGGCagcaUGUCGGAGCGCAa-- -3' miRNA: 3'- ccACUGC---CCGa---ACAGCCUCGCGUcuu -5' |
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13960 | 5' | -57.2 | NC_003521.1 | + | 117515 | 0.67 | 0.876669 |
Target: 5'- cGGUGGCGGGa-UGgCGGcGGCGcCGGGAg -3' miRNA: 3'- -CCACUGCCCgaACaGCC-UCGC-GUCUU- -5' |
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13960 | 5' | -57.2 | NC_003521.1 | + | 29055 | 1.08 | 0.00371 |
Target: 5'- cGGUGACGGGCUUGUCGGAGCGCAGAAa -3' miRNA: 3'- -CCACUGCCCGAACAGCCUCGCGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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