miRNA: 3'- ccaCUGCCCGaaCaGCCUC-GCGUCuu -5'
miRNA: 3'- -CC-ACUGCCCGaacAGCCUCGc-GUCUu -5'
miRNA: 3'- -CC-ACUG-CCCGaa--CAGCcu-CGCGUCUU- -5'
miRNA: 3'- -CCACugCCCGaAC---AGCcu---CGCGUCUu -5'
miRNA: 3'- -CCACUgCCCGaacaGCCU----C-GCGUCUU- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13960 | 5' | -57.2 | NC_003521.1 | + | 202848 | 0.66 | 0.920135 |
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Target: 5'- --cGGCGGGCggGgCGGAGaCGCGGc- -3' miRNA: 3'- ccaCUGCCCGaaCaGCCUC-GCGUCuu -5' |
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| 13960 | 5' | -57.2 | NC_003521.1 | + | 213530 | 0.66 | 0.925463 |
|
Target: 5'- aGGaUGGCGGGCagcUCGGAGCacCAGGu -3' miRNA: 3'- -CC-ACUGCCCGaacAGCCUCGc-GUCUu -5' |
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| 13960 | 5' | -57.2 | NC_003521.1 | + | 103557 | 0.66 | 0.930567 |
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Target: 5'- gGGaUGACgGGGCccagGUCGcccGCGCAGAAg -3' miRNA: 3'- -CC-ACUG-CCCGaa--CAGCcu-CGCGUCUU- -5' |
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| 13960 | 5' | -57.2 | NC_003521.1 | + | 146701 | 0.66 | 0.930567 |
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Target: 5'- aGGUGugGGGCaUGuuaUCGuagccGCGCAGGu -3' miRNA: 3'- -CCACugCCCGaAC---AGCcu---CGCGUCUu -5' |
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| 13960 | 5' | -57.2 | NC_003521.1 | + | 68745 | 0.66 | 0.930567 |
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Target: 5'- gGGUGAgGGGCgg--CGGGuucuGaCGCAGAAa -3' miRNA: 3'- -CCACUgCCCGaacaGCCU----C-GCGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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