Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
13961 | 3' | -53 | NC_003521.1 | + | 104157 | 0.66 | 0.987385 |
Target: 5'- gGUGCUC-UGcGGcGGCGGCgcgGGUCG-Ca -3' miRNA: 3'- -CAUGAGuACuUC-UCGCCGa--CCAGCaG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 196886 | 0.66 | 0.987385 |
Target: 5'- -cGCggGUGAggccGGGGCGGaaggcgGGUCGUCg -3' miRNA: 3'- caUGagUACU----UCUCGCCga----CCAGCAG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 96032 | 0.66 | 0.988848 |
Target: 5'- aGUGUUCAUGAGgaagaggcuGAGgGGCUcGUCGUCc -3' miRNA: 3'- -CAUGAGUACUU---------CUCgCCGAcCAGCAG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 156564 | 0.66 | 0.988848 |
Target: 5'- cGUACUUcgGAGGuuauagucaAGCGGgUGGggGUCa -3' miRNA: 3'- -CAUGAGuaCUUC---------UCGCCgACCagCAG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 212853 | 0.66 | 0.992457 |
Target: 5'- ---aUCcUGggGggcAGCGGUaUGGUCGUCg -3' miRNA: 3'- caugAGuACuuC---UCGCCG-ACCAGCAG- -5' |
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13961 | 3' | -53 | NC_003521.1 | + | 181800 | 0.66 | 0.992457 |
Target: 5'- --cCUCGUGcgucAGGGCGG--GGUCGUCu -3' miRNA: 3'- cauGAGUACu---UCUCGCCgaCCAGCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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