Results 61 - 67 of 67 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13972 | 5' | -55.4 | NC_003521.1 | + | 235903 | 0.66 | 0.951608 |
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Target: 5'- uUCgCGCUCACaCGCCUcGCGAcu-CCUGa -3' miRNA: 3'- uAG-GCGAGUG-GUGGA-UGCUcauGGAC- -5' |
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| 13972 | 5' | -55.4 | NC_003521.1 | + | 145091 | 0.66 | 0.951608 |
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Target: 5'- -gUgGCUCAgCCGCCUGCuGG-ACCUGg -3' miRNA: 3'- uaGgCGAGU-GGUGGAUGcUCaUGGAC- -5' |
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| 13972 | 5' | -55.4 | NC_003521.1 | + | 134943 | 0.66 | 0.951608 |
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Target: 5'- -gCCGuCUUGCgCACCUGCGAGUugaGgCUGa -3' miRNA: 3'- uaGGC-GAGUG-GUGGAUGCUCA---UgGAC- -5' |
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| 13972 | 5' | -55.4 | NC_003521.1 | + | 119875 | 0.67 | 0.949164 |
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Target: 5'- gGUCCGCagucgguucuccucgUCGCC-CC-GCGGGUggGCCUGg -3' miRNA: 3'- -UAGGCG---------------AGUGGuGGaUGCUCA--UGGAC- -5' |
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| 13972 | 5' | -55.4 | NC_003521.1 | + | 111498 | 0.67 | 0.94749 |
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Target: 5'- --gCGCUCaACCGCCUGCuGGGcgACCUc -3' miRNA: 3'- uagGCGAG-UGGUGGAUG-CUCa-UGGAc -5' |
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| 13972 | 5' | -55.4 | NC_003521.1 | + | 44067 | 0.67 | 0.94749 |
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Target: 5'- gAUCCGCaacCcCUACCUGCGGGUcaaGCCg- -3' miRNA: 3'- -UAGGCGa--GuGGUGGAUGCUCA---UGGac -5' |
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| 13972 | 5' | -55.4 | NC_003521.1 | + | 36350 | 0.75 | 0.577321 |
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Target: 5'- cGUCCuaUCACCugacgGCCUACGAGcacUACCUGa -3' miRNA: 3'- -UAGGcgAGUGG-----UGGAUGCUC---AUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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