Results 1 - 20 of 34 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13974 | 3' | -59.7 | NC_003521.1 | + | 58959 | 0.65 | 0.859172 |
Target: 5'- gCUCGCCGaGGGGucgugcguGGCGaaucagccgcuagaGGAGGGGGa -3' miRNA: 3'- aGAGCGGCaCUCCu-------UCGC--------------CCUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 169961 | 0.66 | 0.828437 |
Target: 5'- -gUCGUCGUugcucuccuccuggGAGGcGGCGGcGGGGGAc -3' miRNA: 3'- agAGCGGCA--------------CUCCuUCGCC-CUCCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 39970 | 0.66 | 0.846453 |
Target: 5'- --gCGUCGUGAGGAauugaAGUGGGgaAGGGc- -3' miRNA: 3'- agaGCGGCACUCCU-----UCGCCC--UCCCuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 26034 | 0.66 | 0.822779 |
Target: 5'- --aCGgCGgugaaGAGGAcggcggcgAGCGGGAGGGGGg -3' miRNA: 3'- agaGCgGCa----CUCCU--------UCGCCCUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 118791 | 0.66 | 0.846453 |
Target: 5'- aUCUCGCUGcggagGuAGGAGGUGGGuuucAGGGc- -3' miRNA: 3'- -AGAGCGGCa----C-UCCUUCGCCC----UCCCuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 240198 | 0.66 | 0.846453 |
Target: 5'- --gCGUCGUGAGGAauugaAGUGGGgaAGGGc- -3' miRNA: 3'- agaGCGGCACUCCU-----UCGCCC--UCCCuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 32350 | 0.66 | 0.830837 |
Target: 5'- gUUUGCCG-GAGccGGCGGGAcGGGGc -3' miRNA: 3'- aGAGCGGCaCUCcuUCGCCCU-CCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 45451 | 0.66 | 0.846453 |
Target: 5'- cCUCGCCGUGGcGuGuuGCuGGAuGGGAAg -3' miRNA: 3'- aGAGCGGCACU-C-CuuCGcCCU-CCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 46381 | 0.66 | 0.838731 |
Target: 5'- ---gGCUGUGAGGGGcGCGGGGugcguGGGGc -3' miRNA: 3'- agagCGGCACUCCUU-CGCCCU-----CCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 105469 | 0.66 | 0.846453 |
Target: 5'- --cCGCCGcgGAGacggcgcgcGCGGGAGGGAGg -3' miRNA: 3'- agaGCGGCa-CUCcuu------CGCCCUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 148976 | 0.67 | 0.814564 |
Target: 5'- gUC-CGCCGcccuGGAGGgGGGAuGGGAGa -3' miRNA: 3'- -AGaGCGGCacu-CCUUCgCCCU-CCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 229247 | 0.67 | 0.806197 |
Target: 5'- ---gGCCGcgGAGGugcuuGCGGuGAGGGAAg -3' miRNA: 3'- agagCGGCa-CUCCuu---CGCC-CUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 190686 | 0.67 | 0.806197 |
Target: 5'- aCUaCGCCGgggaGAGGAGaCGGGAGGa-- -3' miRNA: 3'- aGA-GCGGCa---CUCCUUcGCCCUCCcuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 7975 | 0.67 | 0.780267 |
Target: 5'- ---gGCCGgGAGGGuccgcGGCGGcGGGGGAGg -3' miRNA: 3'- agagCGGCaCUCCU-----UCGCC-CUCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 232571 | 0.67 | 0.771371 |
Target: 5'- --cUGCUGUGuGGuGAGCGGGGGGcGAc -3' miRNA: 3'- agaGCGGCACuCC-UUCGCCCUCC-CUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 67258 | 0.67 | 0.771371 |
Target: 5'- -aUCGacCCGUGgcAGGAgGGCGGGcAGGGAGg -3' miRNA: 3'- agAGC--GGCAC--UCCU-UCGCCC-UCCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 105004 | 0.67 | 0.771371 |
Target: 5'- cUUCGCUGcUGcAGG-AGCGGGAacGGGAGc -3' miRNA: 3'- aGAGCGGC-AC-UCCuUCGCCCU--CCCUU- -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 172911 | 0.67 | 0.780267 |
Target: 5'- cCUCGUCcuucuggGAGGAGGaGGGAGGGu- -3' miRNA: 3'- aGAGCGGca-----CUCCUUCgCCCUCCCuu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 91351 | 0.67 | 0.797687 |
Target: 5'- gCUgGUCGgGAGGAcgguGgGGGAGGGGg -3' miRNA: 3'- aGAgCGGCaCUCCUu---CgCCCUCCCUu -5' |
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13974 | 3' | -59.7 | NC_003521.1 | + | 28032 | 0.67 | 0.786422 |
Target: 5'- --aCGCCGcG-GGGAGCGGGAcccaacagaggcggGGGAGa -3' miRNA: 3'- agaGCGGCaCuCCUUCGCCCU--------------CCCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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